20个短串联重复序列基因座杂合度与男性精神分裂症的相关性研究  

Association between the heterozygosity of 20 short tandem repeats loci and male schizophrenia

作　　者：巴华杰[1] 杨春 陆子全 高志勤 赵汉清 BA Huajie;YANG Chun;LU Ziquan(Institute of Forensic Science of Changzhou Municipal Security Bureau,Changzhou 213003,China)

机构地区：[1]常州市公安局物证鉴定所DNA实验室,江苏常州213003 [2]解放军联勤保障部队第九○四医院常州医疗区

出　　处：《精神医学杂志》2022年第6期634-637,共4页Journal of Psychiatry

摘　　要：目的探讨短串联重复序列(STR)基因座杂合度与男性精神分裂症(SCH)的相关性。方法运用PowerPlex 21 System荧光标记复合扩增体系对355例男性SCH患者(研究组)与473名健康男性(对照组)样本的20个STR基因座(D3S1358、D1S1656、D6S1043、D13S317、Penta E、D16S639、D2S1338、D18S51、CSF1PO、TH01、vWA、Penta D、D21S11、D5S818、D8S1179、TPOX、D12S391、D7S820、D19S433、FGA)开展PCR(聚合酶链反应)复合扩增,再针对扩增产物借助AB3500xL型基因分析系统开展电泳与基因测定。对比2个群体内杂合子比率与20个STR基因座在杂合度上的区别。结果两组20个STR基因座的基因型频率观察值与理论值分布皆与Hardy-Weinberg平衡相符(P>0.05)。D3S1358基因座研究组的杂合度高于对照组(P<0.05),其余STR基因座两组的杂合度比较差异均无统计学意义(P>0.05)。结论D3S1358基因座杂合性增大和男性SCH的发病可能有关。Objective To explore the relationship between male schizophrenia and the heterozygosity of 20 short tandem repeats(STRs)loci.Methods The biological samples of 355 male schizophrenics(study group)and 473 healthy males(control group)were collected and amplified by PowerPlex 21 System and separated by electrophoresis to obtain the heterozygosity of 20 STRs loci(D3S1358、D1S1656、D6S1043、D13S317、Penta E、D16S639、D2S1338、D18S51、CSF1PO、TH01、vWA、Penta D、D21S11、D5S818、D8S1179、TPOX、D12S391、D7S820、D19S433、FGA)in both groups.Results All the 20 STRs loci in both groups were found to coincide with Hardy-Weinberg equilibrium(P>0.05).The heterozygosity in study group was significantly higher than that in control group in D3S1358 locus(P<0.05),and the heterozygosity of other STR loci between both groups were not different(P>0.05).Conclusion The improvement in heterozygosity of D3S1358 may be associated with male schizophrenia.

关 键 词：男性 精神分裂症 短串联重复序列 杂合度 

分 类 号：R749.3[医药卫生—神经病学与精神病学]