Construction of an immortalized neural progenitor cell strain and analysis of its immunogenicity  

作　　者：Feng GAO Yuke TIAN Hui YANG Ke AN Ying XU Xuebi TIAN Chuanhan ZHANG 

机构地区：[1]Department of Anesthesiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [2]Department of Anesthesiology,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510180,China [3]Department of Anesthesiology,Children’s Hospital of Chongqing Medical University,Chongqing 400014,China

出　　处：《Frontiers of Medicine》2008年第1期63-69,共7页医学前沿（英文版）

基　　金：The study was supported by the National Natural Science Foundation of China(Grant No.30170905).

摘　　要：Neural progenitor cells(NPC)are those that are the source of neural cells for cell transplantation and gene therapy.The shortage in quantity and the limited life spans of primary cultured cells limit its widespread use in basic research.Immortalized NPC,which also possess the capacity of self-renewal and can proliferate infinitely,can produce a large number of NPCs with stable phenotype and genotype.Here we report that an immortalized neural progenitor cell strain,which we named as INPC,was suc-cessfully established by gene-transfer of simian virus 40 large T antigen gene mediated by liposomes.The INPC retained the biological characteristics of its original cells and provided a safe and reliable cell platform for the treat-ment of central nervous system diseases and transgenic cell transplantation.INPC could express low levels of MHC antigens which was down-regulated after differentiation.This indicates that INPC possesses poor immunogenicity.The immortalized cells may show good long-term survival and do not elicit an acute immunological response follow-ing transplantation.

关 键 词：simian virus 40 antigens polyomavirus trans-forming stem cells 

分 类 号：R73[医药卫生—肿瘤]