HPV感染与宫颈病变患者免疫调节失衡及细胞异常增殖状态的相关性  

Correlation of HPV Infection with Immunoregulatory Imbalance and Abnormal Cell Proliferation in Patients with Cervical Lesions

作　　者：侯颖 李艳梅 李秀兰[1] 刘青[1] HOU Ying;LI Yanmei;LI Xiulan(Beijing You'an Hospital,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京佑安医院妇科,北京100069

出　　处：《河北医学》2023年第8期1274-1279,共6页Hebei Medicine

基　　金：中国中医科学院科研创新工程项目,(编号:C12021A02102)。

摘　　要：目的:探讨人乳头瘤病毒(HPV)感染与宫颈病变患者免疫调节失衡及细胞异常增殖状态的关系。方法:选取2020年1月至2022年4月在我院治疗的高危HPV感染宫颈病变患者56例(高危HPV组),低危HPV感染宫颈病变患者51例(低危HPV组),HPV阴性宫颈病变患者57例(HPV阴性组),同时选取健康体检女性60例(正常组),比较各组细胞及体液免疫指标、细胞因子、p16蛋白、Ki67蛋白、Prdx4 mRNA和STAT3 mRNA差异。结果:高危HPV组、低危HPV组CD4+细胞、免疫球蛋白G(IgG)和免疫球蛋白A(IgA)明显低于HPV阴性组和正常组(P<0.05),且高危HPV组CD4^(+)细胞、IgG和IgA明显低于低危HPV组(P<0.05)。高危HPV组、低危HPV组白细胞介素-2(IL-2)明显低于HPV阴性组和正常组(P<0.05),且高危HPV组IL-2明显低于低危HPV组(P<0.05);高危HPV组、低危HPV组白细胞介素-4(IL-4)、白细胞介素-17(IL-17)和转化生长因子-β(TGF-β)明显高于HPV阴性组和正常组(P<0.05),且高危HPV组IL-4、IL-17和TGF-b明显高于低危HPV组(P<0.05)。高危HPV组、低危HPV组p16、Ki67蛋白阳性表达率明显高于HPV阴性组和正常组(P<0.05)。高危HPV组、低危HPV组Prdx4和STAT3 mRNA相对表达量明显高于HPV阴性组和正常组(P<0.05),且高危HPV组Prdx4和STAT3 mRNA相对表达量明显高于低危HPV组(P<0.05)。结论:HPV感染加重宫颈病变患者免疫调节失衡,促进宫颈细胞异常增殖,尤其在高危型HPV感染中,上述作用更加明显。Objective:To investigate the relationship between human papillomavirus(HPV)infection and immune dysregulation and abnormal cell proliferation in cervical lesions.Methods:In order to investigate this relationship,a study was conducted from January 2020 to April 2022,in which 56 cases of high-risk HPV-infected cervical lesion patients(high-risk HPV group),51 cases of low-risk HPV-infected cervical lesion patients(low-risk HPV group),57 cases of HPV-negative cervical lesion patients(HPV-negative group),and 60 healthy women(normal group)were selected.The cellular and humoral immune indicators,cytokines,p16 protein,Ki67 protein,Prdx4 mRNA,and STAT3 mRNA were compared among the groups.Results:The CD4^(+) cells,immunoglobulin G(IgG),and immunoglobulin A(IgA)levels were significantly lower in the high-risk HPV group and low-risk HPV group than in the HPV-negative group and normal group(P<0.05).Furthermore,the levels of CD4+cells,IgG and IgA were significantly lower in the high-risk HPV group than in the low-risk HPV group(P<0.05).The levels of interleukin-2(IL-2)were significantly lower in the high-risk HPV group and low-risk HPV group than in the HPV-negative group and normal group(P<0.05),with the high-risk HPV group showing significantly lower IL-2 levels than the low-risk HPV group(P<0.05).The levels of interleukin-4(IL-4),interleukin-17(IL-17),and transforming growth factor-β(TGF-β)were significantly higher in the high-risk HPV group and low-risk HPV group than in the HPV-negative group and normal group(P<0.05),with the high-risk HPV group showing significantly higher levels of IL-4,IL-17,and TGF-βthan the low-risk HPV group(P<0.05).The positive expression rates of p16 and Ki67 proteins were significantly higher in the high-risk HPV group and low-risk HPV group than in the HPV-negative group and normal group(P<0.05).The relative expression levels of Prdx4 and STAT3 mRNA were significantly higher in the high-risk HPV group and low-risk HPV group than in the HPV-negative group and normal group(P<0.05),with the high

关 键 词：人乳头瘤病毒 宫颈病变 免疫调节 细胞增殖 

分 类 号：R737.33[医药卫生—肿瘤]