实时荧光PCR法鉴别莜面中掺杂淀粉的植物源性  

Identification Plant-Derived of Naked Oats Adulterated Starch by Real-Time PCR Method

作　　者：陈佳琛 陈晨 张瑞 彭瑛琪 贺丽霞 狄慧 CHEN Jiachen;CHEN Chen;ZHANG Rui;PENG Yingqi;HE Lixia;DI Hui(Zhangjiakou Institute for Food and Drug Control,Zhangjiakou 075000,China;Hebei Food Safety Key Laboratory,Hebei Food Inspection and Research Institute,Shijiazhuang 050071,China)

机构地区：[1]张家口市食品药品检验中心,河北张家口075000 [2]河北省食品检验研究院,河北省食品安全重点实验室,河北石家庄050071

出　　处：《食品安全导刊》2023年第11期122-128,共7页China Food Safety Magazine

基　　金：河北省市场监督管理局科技计划项目(2021ZD35)。

摘　　要：以红薯ACtin基因、马铃薯UGPase基因、玉米ZEIN基因、木薯g3pdh基因设计种属特异性引物探针,以莜麦Avenin基因、真核生物18S rRNA为对照,建立一种实时荧光PCR法对莜面掺杂植物源性淀粉进行鉴别。该方法特异性强,相对灵敏度为0.1%,可用于莜面中掺杂植物源性淀粉的鉴别。Using ACtin gene of sweet potato,UGPase gene of potato,ZEIN gene of maize and g3pdh gene of cassava,species-specific primers were designed,and naked oats Avenin gene and eukaryote 18S rRNA were used as controls,a real-time PCR method was developed for the identification of naked oats adulterated plant-derived starch.The method has high specificity and relative sensitivity of 0.1%.It can be used to identify the adulterated plant-derived starch in naked oats.

关 键 词：实时荧光PCR 植物源性成分 掺杂 

分 类 号：TS211.7[轻工技术与工程—粮食、油脂及植物蛋白工程]