LC-MS/MS测定牛奶中四环素残留量的不确定度评定  

Uncertainty Evaluation for Determination of Tetracycline Residues in Milk by LC-MS/MS

作　　者：冯翀 王小乔 潘秀丽 马建忠[2] FENG Chong;WANG Xiaoqiao;PAN Xiuli;MA Jianzhong(Lanzhou Institute of Food and Drug Inspection/State Key Laboratory of Market Regulation(Monitoring and Control of Pesticide and Veterinary Drug Residues in Food),Lanzhou 730050,China;College of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China)

机构地区：[1]兰州市食品药品检验检测研究院国家市场监管重点实验室(食品中农药兽药残留监控),甘肃兰州730050 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《食品安全导刊》2023年第22期91-95,共5页China Food Safety Magazine

基　　金：甘肃省市场监督管理局技术计划项目(SSCJG-SP-202108)。

摘　　要：目的:分析评定《食品安全国家标准动物性食品中四环素类、磺胺类和喹诺酮类药物残留量的测定液相色谱-串联质谱法》(GB 31658.17—2021)检验方法中牛奶兽药残留四环素测量结果的不确定度。方法:根据《测量不确定度评定与表示》(JJF 1059.1—2012)、参照GB 31658.17—2021方法测定牛奶中四环素含量。通过对牛奶中四环素残留量检测过程中相关不确定度分量进行数学建模,分析评定牛奶中兽药残留四环素测量结果的不确定度。结果:牛奶中四环素测量的扩展不确定度为1.197 4μg·kg-1,牛奶中四环素残留量测定结果为(41.58±1.197 4)μg·kg(k=2)。结论:在四环素的检测中,可通过控制标准溶液的配制过程、增加标准曲线溶液的测量次数和提高仪器的稳定性和灵敏度等方法来提高四环素检测结果的准确性和可靠性。Objective:To analyze and evaluate the uncertainty of the measurement results of tetracycline residue in milk in GB 31658.17—2021 test method.Method:The content of tetracycline in milk was determined according to JJF 1059.1—2012 and GB 31658.17—2021.The uncertainty of the measurement result of tetracycline residue in milk was analyzed and evaluated by mathematical modeling of the correlation uncertainty in the detection process of tetracycline residue in milk.Result:The extended uncertainty of the measurement of tetracycline in milk was 1.1974μg·kg^(-1),and the residual determination of tetracycline in milk was(41.58±1.1974)μg·kg^(-1)(k=2).Conclusion:The accuracy and reliability of tetracycline detection can be improved by controlling the preparation process of the standard solution,increasing the measurement times of the standard curve solution and improving the stability and sensitivity of the instrument.

关 键 词：牛奶 四环素 不确定度 评定 

分 类 号：O657.63[理学—分析化学] TS252.7[理学—化学]