基于聚集诱导发光的多肽荧光探针的制备及其在早期龋齿检测中的应用  

Preparation of a polypeptide fluorescent probe based on aggregation induced luminescence and its application in the detection of early dental caries

作　　者：王冠华 高志远 张晓燕 连小丽 代晓华 颜艳 邹慧儒 丁丹 Wang Guanhua;Gao Zhiyuan;Zhang Xiaoyan;Lian Xiaoli;Dai Xiaohua;Yan Yan;Zou Huiru;Ding Dan(School of Medicine,Nankai University,Tianjin Stomatological Hospital,Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction,Tianjin 300041,China;College of Life Sciences,Nankai University,State Key Laboratory of Medicinal Chemical Biology,Key Laboratory of Bioactive Materials,Ministry of Education,Tianjin 300071.China)

机构地区：[1]南开大学医学院,天津市口腔医院,天津市口腔功能重建重点实验室,天津300041 [2]南开大学生命科学学院,药物化学生物学国家重点实验室,生物活性材料教育部重点实验室,天津300071

出　　处：《国际生物医学工程杂志》2023年第3期185-190,共6页International Journal of Biomedical Engineering

基　　金：天津市卫生健康委科技人才培育项目(KJ20105)

摘　　要：目的:制备基于聚集诱导发光的多肽荧光探针,并对其在早期龋齿检测中的应用进行探讨。方法:将8个天冬氨酸-丝氨酸-丝氨酸(DSS)与聚集诱导发光材料结合制备多肽荧光探针,体外建立人工脱矿模型,将样本浸泡于多肽荧光探针溶液中1 min,应用荧光成像系统对牙齿样本进行检测并收集图像和荧光数据。同时应用扫描电镜观察牙齿的表型,电子显微镜检测牙齿釉质表面钙磷比,偏光显微镜观察牙齿釉质区域。结果:多肽荧光探针处理区域明显可以观察到脱矿牙齿的荧光强度低于正常牙齿,且差异有统计学意义( P<0.05)。扫描电镜显示脱矿组的牙釉质表面可见较多不规则孔隙;未脱矿组的牙釉质表面较为表面平坦,可见一些不规则的片状物堆积。偏振光显微镜结果显示正常牙齿的釉质区域可以观察到明显的双折射,而脱矿牙齿的釉质区域可以观察到一道黑色区域,或是双折射效应消失,可见部分黑色暗影。 结论:制备了一种基于聚集诱导发光的多肽荧光探针,其能够精准定位牙釉质,在早期龋齿检测中具有一定的应用价值。ObjectiveTo prepare a peptide fluorescent probe based on aggregation-induced emission and to investigate its application in the detection of early caries.MethodsEight aspartate-serine-serine(DSS)were combined with aggregation-induced emission material to prepare peptide fluorescent probes,and an artificial demineralization model was established in vitro.The samples were immersed in the peptide fluorescent probe solution for 1 min,and a fluorescence imaging system was applied to examine the tooth samples and collect images and fluorescence data.Scanning electron microscopy was also applied to observe the phenotype of the teeth,and electron microscopy was applied to detect the calcium-phosphorus ratio on the enamel surface of the teeth.Polarized light microscopy was also applied to observe the enamel area of the teeth.ResultsThe fluorescence intensity of demineralized teeth was clearly observed to be lower than that of normal teeth in the peptide fluorescent probe-treated area,and the difference was statistically significant(P<0.05).The results of scanning electron microscopy showed that the enamel surface of the demineralized group had more irregular pores,while the enamel surface of the undemineralized group was flatter with only some irregular accumulation of flakes.The results of polarized light microscopy showed that a clear birefringence could be observed in the enamel region of normal teeth,while a black area or the disappearance of the birefringence effect accompanied by a partial black dark shadow could be observed in the enamel region of demineralized teeth.ConclusionsAn aggregation-induced luminescence-based peptide fluorescent probe was successfully prepared,which can precisely localize the enamel and show some application value in early caries detection.

关 键 词：聚集诱导发光材料 多肽 荧光探针 早期龋检测 

分 类 号：O657.3[理学—分析化学] R781.1[理学—化学]