长链非编码RNA MITA1表达下调对肝癌细胞增殖、迁移和凋亡的影响  

Effect of Down-regulation of Lnc RNA MITA1 on Proliferation,Mi gration and Apoptosis of Hepatoma Cells

作　　者：金雯[1] 王波[1,2] 周健坤 施炜 JIN Wen;WANG Bo;ZHOU Jian-kun;SHI Wei(Department of Medical Nursing,Tongling polytechnic College,Tongling,Anhui 244061;Department of Surgery,Tongling Renhe Hospital,Tongling,Anhui 244021,China)

机构地区：[1]铜陵职业技术学院医学护理系,安徽铜陵244061 [2]铜陵市仁和医院外科,安徽铜陵244021

出　　处：《德州学院学报》2023年第4期12-15,共4页Journal of Dezhou University

基　　金：安徽高校自然科学研究重点项目(KJ2020A0974,2022AH052752);安徽高校优秀青年人才支持计划重点项目(gxyqZD2021155);铜陵职业技术学院自然科学研究重点项目(tlpt2020NK008);铜陵市仁和医院“三新”项目(铜仁医[2021]14号)。

摘　　要：目的:探究LncRNA MITA1表达下调对肝癌细胞迁移和凋亡的影响。方法:采用siRNA调低MITA1表达水平。SMMC7721细胞转染后记为:SMCC7721组、Si-MITA1-NC组、Si-MITA1组。qRT-PCR检测MITA1 mRNA表达;MTT法检测细胞的增殖;细胞划痕实验检测细胞迁移力;流式细胞术检测细胞凋亡率。结果:qRT-PCR检测结果显示,siRNA MITA1转染的SMCC7721细胞株MITA1mRNA表达水平调低(P<0.05);MTT法、细胞划痕实验和流式细胞术检测结果均显示,Si-MITA1组细胞增殖、迁移率降低,细胞凋亡率升高(P<0.05)。结论:调低LncRNA MITA1可以抑制肝癌细胞的进展,MITA1可能为肝癌诊疗潜在的靶点。Objective:To investigate the effect of down-regulation of LncRNA MITA1 on migration and apoptosis of hepatoma cells..Methods:The expression level of MITA1 was down-regulated by siRNA.SMMC7721 cells were divided into three groups:SMCC7721 group,SI-MITA1-NC Group and Si-MITA1 group.The expression of MITA1 mRNA was detected by qRT-PCR,cell proliferation was detected by MTT assay,cell migration was detected by scratch test,and apoptosis was detected by Flow cytometry staining.Results:The expression of MITA1 mRNA in SMCC7721 cells transfected with siRNA-MITA1 was down-regulated by qRT-PCR(P<0.05),and the expression of MITA1 mRNA in SMCC7721 cells transfected with siRNA-MITA1 was also down-regulated by MTT assay,cell scratch test and Flow cytometry assay,in Si-MITA1 group,the cell proliferation and migration rate were decreased,while the cell apoptosis rate was increased(P<0.05).Conclusion:Down-regulation of LncRNA MITA1 can inhibits the progression of liver cancer cells,and MITA1 may be a potential target for liver cancer diagnosis and treatm ent.

关 键 词：长链非编码RNA MITA1 肝细胞癌 增殖 迁移 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]