慢性高眼压青光眼食蟹猴巩膜及筛板生物力学特性的变化  

Changes in biomechanical properties of sclera and lamina cribrosa in cynomolgus monkeys with glaucoma induced by chronic high intraocular pressure

作　　者：吴宛蔓 贾旭 谷浩 陈美龄 龚垚 沈柏宇 李朗 WU Wanman;JIA Xu;GU Hao;CHEN Meiling;GONG Yao;SHEN Boyu;LI Lang(Department of Ophthalmology,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou Province,China)

机构地区：[1]贵州医科大学附属医院眼科,贵州省贵阳市550004

出　　处：《眼科新进展》2023年第9期686-691,共6页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:81960175);贵州省科技计划项目[编号:黔科合基础(2019)1268号];贵州省科学技术厅项目[编号:黔科合平台人才(2017)5718];贵州省卫健委科学技术基金项目(编号:gzwjkj2017-1-042)。

摘　　要：目的探讨慢性高眼压(IOP)青光眼食蟹猴巩膜及筛板(LC)各区域生物力学特性的变化。方法选取健康雄性食蟹猴3只6眼,右眼建立慢性高IOP青光眼模型(实验组),左眼为正常对照眼(对照组)。慢性高IOP青光眼模型建模成功后获取双眼眼球并测量眼球外径,在上方、下方、鼻侧、颞侧4个象限获取视盘周围巩膜(ppSCl)组织试件并测量ppSCl厚度,对各象限ppSCl组织进行双轴拉伸、应力松弛和蠕变试验,并比较两组食蟹猴ppSCl组织弹性模量、应力松弛模量和蠕变率。获取含有巩膜的视盘组织试件并对ppSCl和LC组织进行生物纳米压痕试验,比较两组食蟹猴ppSCl和LC组织压缩模量。结果实验组食蟹猴眼球前后径和左右径均大于对照组(均为P<0.05),实验组食蟹猴ppSCl厚度小于对照组(P<0.001)。实验组食蟹猴下方象限ppSCl横向弹性模量大于轴向,上方、下方2个象限ppSCl横向瞬时模量和上方、下方、鼻侧3个象限ppSCl横向平衡模量均大于轴向(均为P<0.05)。实验组食蟹猴上方、鼻侧、颞侧3个象限ppSCl弹性模量,鼻侧、颞侧2个象限ppSCl瞬时模量,颞侧象限ppSCl平衡模量和4个象限ppSCl蠕变率,两个拉伸方向间比较差异均无统计学意义(均为P>0.05);对照组食蟹猴4个象限ppSCl横向弹性模量、瞬时模量和平衡模量均大于轴向(均为P<0.05),横向蠕变率小于轴向(P<0.05)。实验组食蟹猴ppSCl组织两个拉伸方向的弹性模量、瞬时模量和平衡模量均大于对照组(均为P<0.05),蠕变率均小于对照组(均为P<0.05)。实验组食蟹猴ppSCl组织压缩模量大于对照组(P<0.05),LC压缩模量小于对照组(P<0.05)。结论慢性高IOP青光眼改变了食蟹猴ppSCI及LC的生物力学特性,使ppSCl及LC对高IOP缓冲作用下降,导致视神经盘(ONH)对IOP抵抗力降低,最终造成ONH损伤,从生物力学角度提示为患者设定个性化目标IOP的重要性。Objective To investigate the changes in biomechanical properties of sclera and lamina cribrosa(LC)in cynomolgus monkeys with glaucoma induced by chronic high intraocular pressure(IOP).Methods Among the six eyes from three healthy male cynomolgus monkeys,chronic high IOP-induced glaucoma models were established in the right eyes(experimental group),and the left eyes were used as normal controls(control group).After modeling,the eyeballs of all eyes were removed to measure their outer diameter.Peripapillary sclera(ppSCl)tissues were sampled from the superior,inferior,nasal,and temporal areas to measure their thickness.Biaxial tensile,stress relaxation and creep tests were carried out on ppSCl tissues in each quadrant to obtain and compare their elastic moduli,stress relaxation moduli,and creep rates.Optic disc tissue specimens containing sclera were taken,and the compression moduli of ppSCl and LC tissues were obtained by the nanoindentation test.Results The anterior-posterior and left-right lengths of eyeballs in the experimental group were larger than those in the control group(both P<0.05),while the ppSCl thickness in the experimental group was smaller than that in the control group(P<0.001).In the experimental group,the horizontal elastic modulus of ppSCl in the inferior quadrant,the horizontal instantaneous moduli of ppSCl in the superior and inferior quadrants,and the horizontal equilibrium moduli of ppSCl in the superior,inferior,and nasal quadrants were all larger than those in the axial direction(all P<0.05).No significant differences were found in the elastic modulus of ppSCl in the superior,nasal,and temporal quadrants,the instantaneous modulus of ppSCl in the nasal and temporal quadrants,the equilibrium modulus of ppSCl in the temporal quadrant,and the creep rate in the four quadrants in both the horizontal and axial directions in the experimental group(all P>0.05).The horizontal elastic,instantaneous,and equilibrium moduli of ppSCl in the four quadrants in the control group were higher than those in th

关 键 词：青光眼 巩膜 筛板 双轴拉伸 纳米压痕 食蟹猴 

分 类 号：R775[医药卫生—眼科]