虎杖苷在白内障大鼠晶状体上皮细胞损伤中的作用机制  

Effect of polydatin on lens epithelial cell injury in rats with cataract

作　　者：冯冰 梁明明 钟祖斌 FENG Bing;LIANG Mingming;ZHONG Zubin(Department of Ophthalmology,Zhongda Hospital Affiliated to Southeast University,Nanjing 210000,Jiangsu Province,China)

机构地区：[1]东南大学附属中大医院眼科,江苏省南京市210000

出　　处：《眼科新进展》2023年第9期692-696,共5页Recent Advances in Ophthalmology

基　　金：江苏省自然科学基金项目(编号:BK20205870)。

摘　　要：目的探究虎杖苷在白内障大鼠晶状体上皮细胞(LEC)损伤中的作用机制。方法将50只新生大鼠随机分为对照组、模型组、虎杖苷低剂量组、虎杖苷高剂量组、激活剂组,每组10只,除对照组外,其余各组大鼠通过皮下注射亚硒酸钠建立白内障大鼠模型。第2天虎杖苷低、高剂量组大鼠分别使用1.3 g·L^(-1)和5.0 g·L^(-1)虎杖苷滴眼液滴眼,激活剂组大鼠使用5.0 g·L^(-1)虎杖苷滴眼液滴眼外还需每天灌胃10 mg·kg^(-1)SRI-011381盐酸盐,对照组和模型组大鼠使用生理盐水滴眼。裂隙灯生物显微镜检查大鼠晶状体混浊度;HE染色观察大鼠晶状体病理变化;检测大鼠LEC中总超氧化物歧化酶(SOD)和丙二醛(MDA)水平;流式细胞术检测大鼠LEC凋亡情况;Western blot检测大鼠LEC中转化生长因子-β(TGF-β)、Smad2、Smad3、α-平滑肌肌动蛋白(α-SMA)、B淋巴细胞瘤-2(Bcl-2)蛋白及其相关蛋白Bax的表达。结果对照组大鼠晶状体前囊区域结构清晰,LEC与晶状体纤维细胞排列紧密。与对照组相比,模型组大鼠晶状体前囊区域结构紊乱,晶状体纤维细胞变性、肿胀,LEC排列稀疏,晶状体后囊附近发生空泡化,大鼠晶状体混浊度分级、LEC中MDA水平、细胞凋亡率以及TGF-β、Smad2、Smad3、α-SMA、Bax蛋白表达均明显升高(均为P<0.05),LEC中SOD水平以及Bcl-2蛋白表达均明显降低(均为P<0.05)。与模型组相比,虎杖苷低、高剂量组大鼠晶状体结构明显改善,晶状体混浊度分级、LEC中MDA水平、细胞凋亡率以及TGF-β、Smad2、Smad3、α-SMA、Bax蛋白表达均明显降低(均为P<0.05),LEC中SOD水平以及Bcl-2蛋白表达均明显升高(均为P<0.05)。与虎杖苷高剂量组相比,激活剂组大鼠晶状体结构损伤明显加重,晶状体混浊度分级、LEC中MDA水平、细胞凋亡率以及TGF-β、Smad2、Smad3、α-SMA、Bax蛋白表达均明显升高(均为P<0.05),LEC中SOD水平以及Bcl-2蛋白表达均明显降低(均为P<0.Objective To investigate the effect of polydatin on lens epithelial cell(LEC)injury in rats with cataract.Methods A total of 50 newborn rats were randomly divided into the control group,model group,low-dose polydatin group,high-dose polydatin group,and activator group,with 10 rats in each group.Except for the control group,rats in the rest groups were injected with sodium selenite subcutaneously to establish the cataract rat models.Since the second day,rats in the low-and high-dose polydatin groups were given eye drops containing 1.3 g·L^(-1) and 5.0 g·L^(-1) polydatin,respectively;in addition to 5.0 g·L^(-1) polydatin eye drops,rats in the activator group were also given the SRI-011381 hydrochloride(10 mg·kg^(-1))intragastrically;rats in the control and model groups were given normal saline in eyes.The lens opacity was examined by a slit lamp.Hematoxylin-eosin(HE)staining was performed to monitor the pathological changes in the lens.The total superoxide dismutase(SOD)and malondialdehyde(MDA)levels in LECs were measured.The LEC apoptosis was detected by flow cytometry.The expression levels of transforming growth factor-β(TGF-β),Smad2,Smad3,αsmooth muscle actin(α-SMA),B-cell lymphoma-2(Bcl-2),and Bcl-2-associated X(Bax)in LECs were measured by Western blot.Results The anterior capsule of rats in the control group was structurally clear,and LECs and lens fiber cells were closely arranged.Compared with the control group,the model group had structural disorder in the anterior capsule,degeneration and swelling in lens fiber cells,loose LEC arrangement,and vacuolization near the posterior capsule;besides,the lens opacity grade,the LEC apoptosis,and the expression levels of MDA,TGF-β,Smad2,Smad3,α-SMA,and Bax in the model group increased greatly(all P<0.05),and the expression levels of SOD and Bcl-2 decreased greatly(both P<0.05).Compared with the model group,the lens structure of rats in the low-and high-dose polydatin groups improved significantly,the lens opacity grade,the LEC apoptosis,and the expression l

关 键 词：虎杖苷 TGF-β/Smads信号通路 白内障 晶状体上皮细胞损伤 

分 类 号：R776[医药卫生—眼科]