Cloning,Expressing and Characterizing a Novel Cold-Adapted Laminarinase from Marine Bacterium Wenyingzhuangia aestuarii OF219  

作　　者：LI Jiajing XUE Changhu CHANG Yaoguang SHEN Jingjing ZHANG Yuying 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Drugs and Bioproducts,Qingdao Marine Science and Technology Center,Qingdao 266237,China

出　　处：《Journal of Ocean University of China》2023年第4期1034-1040,共7页中国海洋大学学报（英文版）

基　　金：supported by the National Key R&D Program of China(No.2018YFC0311203);the Fundamental Research Funds for the Central Universities(No.201941005).

摘　　要：Laminarinases reveal potential application in the field of food and biotechnology.In this research,a novel GH16 family laminarinase,designated as Lam16A_Wa,was cloned from the genome of marine bacterium Wenyingzhuangia aestuarii OF219 and expressed in Escherichia coli.Lam16A_Wa demonstrates a relatively low optimal reaction temperature(35℃)and a cold-adapted feature.Its optimal pH value is 6.0 and is stable in a broad pH range from 3.0 to 11.0.A glycomics strategy was employed to investigate the hydrolytic pattern of Lam16A_Wa.The enzyme was confirmed as a random endo-acting glycoside hydrolase.Its minimum substrate was laminarin pentasaccharide,and the major final products are oligosaccharides,including disaccharide to pentasaccharide.The Lam16A_Wa provides a novel and well-defined tool for the molecular tailoring of laminarin.

关 键 词：laminarinase LAMINARIN GLYCOMICS GH16 family 

分 类 号：S917.3[农业科学—水产科学]