Comparative chemical characters of Pseudostellaria heterophylla from geographical origins of China  

作　　者：Miao Sha Xiaohuan Li Yu Liu Hongyue Tian Xu Liang Xia Li Wenyuan Gao 

机构地区：[1]Tianjin Key Laboratory for Modern Drug Delivery&High-Efficiency,School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300193,China

出　　处：《Chinese Herbal Medicines》2023年第3期439-446,共8页中草药（英文版）

基　　金：supported by the National Key R&D Program of China(No.2019YFC1710604);the National Key Research and Development Program(No.2020YFA0907903);the key Project at Central Government Level(No.2060302);Tianjin Science and Technology Plan Project(No.19YFZCSY00170);the National Natural Science Foundation of China(No.81872956)。

摘　　要：Objective:Pseudostellaria heterophylla has been paid more attention in recent years,mainly as a medicine food homology plant.The content determination of P.heterophylla is not specified in the Chinese Pharmacopoeia(version 2020).The environmental conditions in different production areas could exert an influence on the quality of P.heterophylla.The purpose of this study is to discriminate P.heterophylla collected from different geographical origins of China.Methods:In this study,the content of polysaccharide in 28 batches of P.heterophylla was determined using phenol–sulfuric acid.HPLC fingerprints were established under optimised HPLC-PDA methods.Subsequently,the similarity analysis(SA)and the quantification of heterophyllin B were analyzed.The metabolites of P.heterophylla were identified and evaluated using UHPLC-Q Exactive HF orbitrap MS system.Principal component analysis(PCA),partial least squares discriminant analysis(PLS-DA),hierarchical cluster analysis(HCA)and orthogonal PLS-DA(OPLS-DA)were performed based on all peak areas.Results:The polysaccharide content in Guizhou and Jiangsu was higher than that of other production areas,which varied significant from different origins.While the content of heterophyllin B in Anhui and Jiangsu was high.The correlation coefficients of HPLC fingerprints for 28 batches samples ranged from 0.877 to 0.990,and the characteristic map can be used to identify and evaluate the quality of P.heterophylla.The samples from Fujian,Guizhou,Jiangsu provinces can be relatively separated using multivariate statistical analysis including PCA,PLS-DA,HCA,OPLS-DA,indicating that their metabolic compositions were significantly different.Ultimately,a total of 15 metabolites which were filtrated by a VIPvalue>1 and a P-value<0.05 associated with the separation of different origins were identified.Conclusion:HPLC fingerprint was established to evaluate the quality and authenticity of P.heterophylla.The present work showed that the difference of geographic distributions had an influence on th

关 键 词：HPLC fingerprint METABOLITES Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm POLYSACCHARIDE UHPLC-Q Exactive HF orbitrap MS system 

分 类 号：R284.1[医药卫生—中药学]