长链非编码RNA DSCAS对肺鳞状细胞癌SK-MES-1细胞增殖及侵袭的影响  

Effects of long non-coding RNA DSCAS on proliferation and invasion of lung squamous cell carcinoma SK-MES-1 cells

作　　者：刘宏平 卢春亚 李萍[1] 贾红霞[1] 王琰[1] 程玖玲 程瑞瑞[1] 张国俊[1] LIU Hongping;LU Chunya;LI Ping;JIA Hongxia;WANG Yan;CHENG Jiuling;CHENG Ruirui;ZHANG Guojun(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院呼吸与危重症医学科,郑州450052

出　　处：《郑州大学学报（医学版）》2023年第4期507-510,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(81874042)。

摘　　要：目的:探讨长链非编码RNA(LncRNA)DSCAS对肺鳞状细胞癌(肺鳞癌)SK-MES-1细胞增殖及侵袭的影响。方法:采用实时荧光定量PCR(qRT-PCR)检测63例肺鳞癌组织及癌旁正常组织中DSCAS的表达。分别设计合成DSCAS过表达(oe-DSCAS)和DSCAS过表达阴性对照(oe-NC),DSCAS短发卡RNA(sh-DSCAS)和DSCAS短发卡RNA阴性对照(sh-NC)4种序列,利用慢病毒感染技术构建相应稳定感染的细胞株,qRT-PCR技术检测DSCAS的表达。通过CCK-8法和克隆形成实验检测细胞的增殖能力,Transwell实验检测细胞的侵袭能力。结果:肺鳞癌组织中DSCAS表达水平高于对应的癌旁正常组织(P<0.001)。oe-DSCAS组DSCAS的表达高于oe-NC组,sh-DSCAS组DSCAS的表达低于sh-NC组(P<0.05)。与oe-NC组比较,oe-DSCAS组的细胞克隆形成数增多,细胞增殖能力升高,侵袭细胞数增多(P<0.05)。与sh-NC组比较,sh-DSCAS组的细胞克隆形成数减少,细胞增殖能力降低,侵袭细胞数降低(P<0.05)。结论:DSCAS可增加肺鳞癌SK-MES-1细胞的增殖和侵袭能力。Aim:To investigate the effects of long non-coding RNA(LncRNA)DSCAS on the proliferation and invasion of lung squamous cell carcinoma SK-MES-1 cells.Methods:qRT-PCR was used to detect the expression of DSCAS in 63 cases of lung squamous cell carcinoma and adjacent normal tissue.Four sequences of DSCAS overexpression(oe-DSCAS),DSCAS overexpression control(oe-NC),DSCAS shRNA(sh-DSCAS),DSCAS shRNA control(sh-NC)were designed and synthesized,respectively.Lentiviral infection technology was used to construct 4 groups of stable infected SK-MES-1 cell lines of DSCAS.qRT-PCR was used to detect the expression of DSCAS in the 4 groups.The proliferation ability of the cells was detected by CCK-8 assay and colony formation assay,and the invasion ability of the cells was detected by Transwell assay.Results:The expression level of DSCAS in the lung squamous cell carcinoma tissue was higher than that in the adjacent normal tissue(P<0.001).The expression of DSCAS in the oe-DSCAS group was significantly higher than that in the oe-NC group(P<0.05),which was lower in the sh-DSCAS group than that in the sh-NC group(P<0.05).compared with those in the oe-NC group,the number of clones formed,cell proliferation ability and invasive cell count increased in the oe-DSCAS group(P<0.05);compared with those in the sh-NC group,the number of clones formed,cell proliferation ability and invasive cell count decreased in the sh-DSCAS group(P<0.05).Conclusion:DSCAS could increase the proliferation and invasion of lung squamous cell carcinoma SK-MES-1 cells.

关 键 词：肺鳞状细胞癌 LncRNA DSCAS 增殖 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]