九里香全长转录组测序分析  

Sequencing Analysis of Full-Length Transcriptome of Murraya exotica L.Mant.

作　　者：梁细妹 梁莹[1] 韦坤华[1] 秦双双[1] 胡营[1] 韦桂丽 韦筱媚[1] 韦范[1] 缪剑华[1,2] LIANG Xi-mei;LIANG Ying;WEI Kun-hua;QIN Shuang-shuang;HU Ying;WEI Gui-li;WEI Xiao-mei;WEI Fan;MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;Guangxi University of Traditional Chinese Medicine,Nanning 530299,China)

机构地区：[1]广西壮族自治区药用植物园/广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,广西南宁530023 [2]广西中医药大学,广西南宁530299

出　　处：《中国现代中药》2023年第7期1505-1514,共10页Modern Chinese Medicine

基　　金：广西创新驱动发展专项(桂科AA18242040);广西自然科学基金项目(2020GXNSFBA159018,2020GXNSFAA297069);国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202005);广西农业科技创新联盟项目(桂农科盟202211);科技基础资源调查专项(2018FY100700);对发展中国家科技援助项目(KY201904001);广西科技计划项目(桂科AD22080012)。

摘　　要：目的:获得九里香Murraya exotica L.Mant.全长转录组数据库,为深入挖掘其全长基因信息提供参考。方法:利用Pacino Sequel平台的第三代测序技术,对九里香的根、茎、叶、花、果混合样品进行全长转录组测序,并对原始转录组数据进行分析。结果:共获得94435个转录本,平均长度为76476.23 bp,80583条环形一致性序列,通过聚类、校正和去冗余后最终得到108443个高质量的isoforms,其中在非冗余蛋白(NR)、核苷酸序列(NT)、基因本体(GO)、真核生物相邻类的聚簇(KOG)、蛋白家族(Pfam)、京都基因与基因组百科全书(KEGG)和SwissProt共注释了100219个isoforms,同时检测出34182个简单序列重复(SSR)位点,其中18482个单核苷酸数量占主导位置;检测了2574个转录因子,29592个长链非编码RNAs(lncRNAs)和30322个mRNAs。结论:获得了较为可靠的九里香全长转录组数据,为深入研究九里香的生长发育、相关代谢途径、功能基因利用、基原物种鉴定等提供数据参考。Objective:To obtain the full-length transcriptome database of Murraya exotica L.Mant.and provide a reference for deeply exploring its full-length gene information.Methods:The third-generation sequencing technology using the Pacino Sequel platform was used to sequence the full-length transcriptome of mixed samples from the root,stem,leaf,flower,and fruit of M.exotica and analyze the original transcriptome data.Results:A total of 94435 transcripts with an average length of 76476.23 bp and 80583 circular consensus sequences were obtained.Finally,108443 high-quality isoforms were obtained after clustering,correcting,and redundancy removing,among which 100219 isoforms were annotated in the databases of Non-Redundant Protein Sequence(NR),Nucleotide(NT),Gene Ontology(GO),Eukaryotic Orthologous Group(KOG),Pfam,Kyoto Encyclopedia of Genes and Genomes(KEGG),and SwissProt.Meanwhile,34182 SSR sites were detected,among which 18482 single nucleotides were in a dominant position.In addition,2574 transcription factors,29592 lncRNAs,and 30322 mRNAs were detected.Conclusion:Reliable full-length transcriptome data of M.exotica were obtained,which provide a reference for further research on the growth,related metabolic pathways,functional gene utilization,and genetic species identification of M.exotica.

关 键 词：九里香 单分子实时测序技术 简单序列重复标记 功能注释 

分 类 号：R282[医药卫生—中药学]