A T follicular helper cell origin for T regulatory type 1 cells  

作　　者：Patricia Solé Jun Yamanouchi Josep Garnica Muhammad Myn Uddin Robert Clarke Joel Moro Nahir Garabatos Shari Thiessen Mireia Ortega Santiswarup Singha Debajyoti Mondal César Fandos Julio Saez-Rodriguez Yang Yang Pau Serra Pere Santamaria 

机构地区：[1]Institut D'investigacions Biomediques August Pi i Sunyer,Barcelona,Spain [2]Department of Microbiology,Immunology and Infectious Diseases,Snyder Institute for Chronic Diseases,Cumming School of Medicine,University of Calgary,Calgary,AB,Canada [3]Institute of Computational Biomedicine,Faculty of Medicine,Heidelberg University Heidelberg,Germany [4]Department of Biochemistry and Molecular Biology,Cumming School of Medicine,University of Calgary,Calgary,AB,Canada.

出　　处：《Cellular & Molecular Immunology》2023年第5期489-511,共23页中国免疫学杂志（英文版）

基　　金：the Canadian Instutes of Health Research(CIHR)(FDN-353029,PJT-479040,PJT-479038,FRN-168480(with JDRF),DT4-179512);Genome Canada(GAPP program),the Praespero Foundation,the Alberta Diabetes Foundation,theISClll and FEDER(PIE14/00027,Pl15/0797);Ministerio de Ciencia e Innovacion of Spain(MCIN;PID2021-125493OB-I00);Generalitat de Catalunya(SGR and CERCA Programmes)and Red Espanola de Supercomputacion(RES,providing CSUC resources).P.Serra was an investigator of the Ramon y Cajal reintegration program and was supported by a JDRF Career Development Award.P.Sole and J.Garnica were supported by predoctoral studentships from FPU(MCIN).

摘　　要：Chronic antigenic stimulation can trigger the differentiation of antigen-experienced CD4+T cells into T regulatory type 1(TR1)cells,a subset of interleukin-10-producing Treg cells that do not express FOxP3.The identities of the progenitor(s)and transcriptional regulators of this T-cell subset remain unclear.Here,we show that the peptide-major histocompatibility complex class Il(pMHCll)monospecific immunoregulatory T-cell pools that arise in vivo in different genetic backgrounds in response to pMHCll-coated nanoparticles(pMHCll-NPs)are invariably comprised of oligoclonal subpools of T follicular helper(TFH)and TR1 cells with a nearly identical clonotypic composition but different functional properties and transcription factor expression profles.Pseudotime analyses of scRNAseq data and multidimensional mass cytometry revealed progressive downregulation and upregulation of TFH and TR1 markers,respectively.Furthermore,pMHCIl-NPs trigger cognate TR1 cell formation in TFH cell-transfused immunodeficient hosts,and T-cell-specific deletion of Bcl6 or Irf4 blunts both the TFH expansion and TR1 formation induced by pMHCl-NPs.In contrast,deletion of Prdm1 selectively abrogates the TFH-to-TR1 conversion.Bcl6 and Prdm1 are also necessary for anti-CD3 mAbinduced TR1 formation.Thus,TFH cells can differentiate into TR1 cells in vivo,and BLIMP1 is a gatekeeper of this cellular reprogramming event.

关 键 词：T-regulatory type 1(TR1)cells autoimmunity T follicular helper(TFH)cells TRANSDIFFERENTIATION nanomedicine 

分 类 号：R392[医药卫生—免疫学]