B(A)血型的鉴定及其输血策略分析  

Identification of B(A)subtype and analysis of bood transfusion strategiaes

作　　者：刘春霞[1] 牛路 柴翠翠 王晗 张红良[1] 高宏军 LIU Chunxia;NIU Lu;CHAI Cuicui;WANG Han;ZHANG Hongliang(Department of Blood Transfusion.The First Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一医院输血科 [2]江阴市力博医药生物技术研究所

出　　处：《中国输血杂志》2023年第8期687-689,共3页Chinese Journal of Blood Transfusion

摘　　要：目的探讨B(A)血型患者的血清学及其基因型鉴定分析方法。方法应用试管法(血清学)对3名患者的临床疑难ABO血型标本做ABO血型正反定型;应用实时荧光定量PCR技术对3例标本做ABO血型基因分型,并采用基因测序方法对其ABO基因1-7外显子测序确定基因型。结果3名患者血清学ABO血型正反定型均表现为“B(A)血型”的格局;3例ABO基因分型均为B/O2,测序均在第7外显子B等位基因基础上发生c.640A>G突变,符合ABO*BA.04基因型特点。结论对于ABO血型亚型等疑难血型的鉴定,采用血清学和基因检测相结合的方法可以准确鉴定血型,保证临床用血安全。Objective To investigate the serology and genotype identification method of B(A)subtype patients.Methods Test tube method(serology)was used to confirm the clinically difficult ABO blood group samples of 3 patients with ABO blood group;ABO blood group was genotyped by real-time PCR,and the ABO gene exon 1-7 was sequenced to determine the genotype.Results The forward and reverse blood typing result of three patients was B(A)subtype all with ABO genotype B/O2 and c.640A>G mutation on B allele of exon 7,which meets the characteristics of ABO∗BA.04 genotype.Conclusion The combination of serological and genetic testing could identify difficult blood types such as ABO subtypes accurately and ensure the safety of clinical blood use.

关 键 词：B(A)型 ABO血型亚型 正反定型 基因定型 基因测序 血清学方法 试管法 PCR 

分 类 号：R457.11[医药卫生—治疗学] Q503[医药卫生—临床医学]