亚麻木酚素对力竭训练大鼠的抗疲劳作用及AMPK通路的影响  

Effects of Flax Lignans on Fatigue Resistance and AMPK Pathway in Exhaustive Exercise-induced Fatigue Rats

作　　者：周蕾[1] 贺帅[1] 席海燕[1] 白慧敏[1] 闫艳[1] 陈建芳[1] Zhou Lei;He Shuai;Xi Haiyan;Bai Huimin;Yan Yan;Chen Jianfang(School of Basic Medicine and Forensic Medicine,Baotou Medical College,Baotou,014040)

机构地区：[1]包头医学院基础医学与法医学院,包头014040

出　　处：《分子植物育种》2023年第17期5809-5816,共8页Molecular Plant Breeding

基　　金：内蒙古卫健委基金项目(2022013771);包头市卫健委基金项目(wsjkwkj012)共同资助。

摘　　要：本研究旨在探究亚麻木酚素(SDG)对力竭训练诱导的运动性疲劳(EIF)大鼠的抗疲劳作用及其机制。通过将大鼠分为CON组、EIF组、L-SDG组、M-SDG组、H-SDG组和H-SDG+Compound C组,进行4周的实验处理。实验结果显示,与EIF组相比,L-SDG组、M-SDG组和H-SDG组的力竭游泳时间增加,血清心肌损伤指标降低,肝糖原、肌糖原和血糖水平增加,乳酸和尿素氮水平降低,骨骼肌超氧化物歧化酶(SOD)和催化酶(CAT)水平增加,骨骼肌丙二醛(MDA)水平降低,骨骼肌AMPKα的磷酸化水平增加,骨骼肌能量代谢相关基因的表达增加(P<0.05)。与H-SDG组相比,H-SDG+Compound C组的力竭游泳时间减少,血清心肌损伤指标上升,肝糖原、肌糖原和血糖水平降低,乳酸和尿素氮水平升高,骨骼肌SOD、CAT和MDA水平增加,骨骼肌AMPKα的磷酸化水平降低,骨骼肌能量代谢相关基因的表达降低(P<0.05)。本研究结果表明,亚麻木酚素通过激活AMPK通路改善力竭训练大鼠的运动能力、抗氧化能力和能量代谢方式,具有抗运动性疲劳的潜力。This study aimed to investigate the anti-fatigue effects of secoisolariciresinol diglucoside(SDG) and its underlying mechanisms in exhaustive exercise-induced fatigue(EIF) rats.Rats were randomly divided into six groups:CON,EIF,L-SDG,M-SDG,H-SDG,and H-SDG+Compound C.The rats underwent a 4-week experimental protocol.Results showed that compared to the EIF group,the L-SDG,M-SDG,and H-SDG groups exhibited prolonged exhaustive swimming time,decreased serum markers of myocardial injury,increased hepatic and muscular glycogen levels,elevated blood glucose levels,reduced lactate and blood urea nitrogen levels,enhanced activities of superoxide dismutase(SOD) and catalase(CAT) in skeletal muscles,decreased malondialdehyde(MDA) levels in skeletal muscles,increased phosphorylation of AMP-activated protein kinase alpha(AMPKα) in skeletal muscles,and upregulated expression of energy metabolism-related genes in skeletal muscles(P0.05).In comparison to the H-SDG group,the H-SDG+Compound C group exhibited reduced exhaustive swimming time,elevated serum markers of myocardial injury,decreased hepatic and muscular glycogen levels,increased blood glucose levels,elevated lactate and blood urea nitrogen levels,elevated SOD,CAT,and MDA levels in skeletal muscles,decreased phosphorylation of skeletal muscle AMPKα,and downregulated expression of energy metabolism-related genes in skeletal muscles(P0.05).These findings indicate that SDG exerts anti-fatigue effects in EIF rats by activating the AMPK pathway,improving exercise performance,enhancing antioxidant capacity,and modulating energy metabolism.

关 键 词：亚麻(Linum usitatissimum) 亚麻木酚素 运动性疲劳 抗氧化能力 能量代谢 

分 类 号：TS201.4[轻工技术与工程—食品科学]