延胡索转录组表达序列标签微卫星标记的开发及其功能分析  

Development and function analysis of EST-SSR markers in the transcriptome of Corydalis yanhusuo

作　　者：张琪 李浩玲 王春花 史若彤 蒋雪梅 祁俊生 周浓 石汝杰 ZHANG Qi;LI Haoling;WANG Chunhua;SHI Ruotong;JIANG Xuemei;QI Junsheng;ZHOU Nong;SHI Rujie(Chongqing Engineering Laboratory of Green Planting and Deep Processing of Famous-region Drug(Three Gorges Reservoir Region),College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing,404100 P.R.China;Huangjiang Middle School of Dongguan,Dongguan,Guangdong,523750 P.R.China;College of Animal Medicine,Hunan Agricultural University,Changsha,Hunan,410125 P.R.China;College of Environmental and Chemistry Engineering,Chongqing Three Gorges University,Chongqing,404100 P.R.China)

机构地区：[1]重庆三峡学院生物与食品工程学院三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404100 [2]东莞市黄江中学,广东东莞523750 [3]湖南农业大学动物医学院,湖南长沙410125 [4]重庆三峡学院环境与化学工程学院,重庆404100

出　　处：《华西药学杂志》2023年第4期399-404,共6页West China Journal of Pharmaceutical Sciences

基　　金：重庆三峡学院青年项目基金(20200081);三峡库区道地药材绿色种植与深加工重庆市工程实验室开放基金(061800107);重庆市渝东北特色生物资源开发利用工程技术研究中心开放基金(20200115)。

摘　　要：目的研究延胡索块茎和叶转录组水平上表达序列标签微卫星(EST-SSR)的分布规律,分析其差异性,并探索含EST-SSR基因的生物学功能,为延胡索遗传多样性评估和遗传育种提供方法和依据。方法利用生物信息学方法构建延胡索块茎和叶的转录组EST-SSR序列库,识别和搜索其EST-SSRs序列,利用TBtools进行GO功能分析,使用KAAS进行KEGG通路富集分析。结果延胡索的块茎和叶转录组中分别有7952个和137197个EST-SSR位点,其序列长度占其转录组长度的比例分别为0.87%、0.89%,丰度分别为475.76、458.91个·Mb^(-1)。延胡索块茎和叶转录组均为三碱基重复类型最多,其中,以AAG/CTT重复类型最多。通过对延胡索块茎含有EST-SSR的unigene进行功能富集分析表明:含EST-SSR的unigene序列的差异表达基因与化合物结合功能、有机物代谢功能、核糖体功能、氧化磷酸化等通路有关。结论延胡索块茎和叶转录组EST-SSR的比例、丰度和密度高度一致,其中,含EST-SSR的unigene具有显著的生物学功能。OBJECTIVE To study distribution patterns of EST-SSR sequence at the transcriptomic level of tuber and leaves of Corydalis yanhusuo,and to explore the differences and biological functions of unigene containing EST-SSR,so as to provide methods and basis for genetic diversity assessment and genetic breeding of C.yanhusuo.METHODS The EST-SSR sequence library of the transcriptome of tuber and leaves of C.yanhusuo was constructed by bioinformatics method,and EST-SSR sequences were identified and searched.TBtools was used for GO function analysis,and KAAS was used for KEGG pathway enrichment analysis.RESULTS There were 7952 and 137197 EST-SSR loci in the tuber and leaf transcriptome of C.yanhusuo,respectively,and the sequence length accounted for 0.87%and 0.89%of the length of transcription,and the abundance was 475.76 and 458.91/Mb,respectively.The transcriptome of tuber and leaf of C.yanhusuo had the highest number of triple base repeats,in which,AAG/CTT is the most repetitive type.The functional enrichment analysis of unigene containing EST-SSR in tuber showed that unigene containing EST-SSR were related to compound binding function,organic metabolic function,ribosome function,oxidative phosphorylation and other pathways.CONCLUSION The ratio,abundance and density of EST-SSR in the transcripts of tubers and leaves of C.yanhusuo are highly consistent,and unigene containing EST-SSR has significant biological function.

关 键 词：延胡索 块茎 叶 转录组 表达序列标签微卫星 单基因 碱基 重复 功能分析 

分 类 号：R28[医药卫生—中药学]