LC-MS分析CmSnf1基因过表达对蛹虫草代谢组的影响  

LC-MS investigation of the metabolomic changes after overexpression of CmSnf1 gene in Cordyceps militaris

作　　者：王晓军 周湘[1] 李乐[2] 李芳盛 荣丹 邹根 汪滢 WANG Xiaojun;ZHOU Xiang;LI Le;LI Fangsheng;RONG Dan;ZOU Gen;WANG Ying(School of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Southern Key Laboratory of Edible Fungi Resource Utilization of Ministry of Agriculture and Rural Affairs,National Engineering Technology Research Center of Edible Fungi,Institute of Edible Fungi of Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]浙江农林大学林业与生物技术学院,浙江杭州311300 [2]上海市农业科学院食用菌研究所、农业农村部南方食用菌资源利用重点实验室、国家食用菌工程技术研究中心,上海201403

出　　处：《菌物学报》2023年第8期1744-1754,共11页Mycosystema

基　　金：国家自然科学基金(31870637,31902089);上海市科学技术委员会“一带一路”国际合作项目(20310741900);上海市农业科学院卓越团队(2022014)。

摘　　要：Sucrose-nonfermenting 1(SNF1)蛋白激酶调节大量基因的转录,改变代谢酶的活性,并控制各种营养反应性细胞发育过程。本研究通过LC-MS非靶向代谢组学技术比较蛹虫草Cordyceps militaris野生型菌株Cm01和CmSnf1基因过表达菌株菌丝体的化学成分,从整体代谢物水平阐明CmSnf1基因过表达对蛹虫草代谢产物积累的影响。研究表明:CmSnf1基因过表达后,在LC-MS正负离子模式下检测出547种差异代谢物,同野生型菌株相比,220种产物上调和327种下调。尤其显著的是黄酮类物质2-香叶基-3,4,2′,4′-四羟基二氢查耳酮上调了140.55倍,甘油磷脂代谢中的磷脂酰丝氨酸(PS)下调了47.53倍。差异代谢产物参与甘油脂代谢、氨基酸生物合成和降解、半乳糖代谢、丙酮酸和酮类化合物代谢等多种途径。CmSnf1过表达菌株与野生型相比在酮类化合物代谢上具有优势,推测CmSnf1基因在蛹虫草药用功效中发挥着重要的作用。Sucrose-nonfermenting 1(SNF1)protein kinase regulates the transcription of a number of genes,alters the activity of metabolic enzymes,and controls various nutrient responsive cell development.In this study,the chemical constituents of Cordyceps militaris wild-type strain Cm01 and CmSnf1 overexpressing strain Cmsnf1 were compared by LC-MS nontargeted metabolomic analysis.The results showed that after CmSnf1 gene overexpression,547 differential metabolites were detected in positive and negative ion mode,showing 220 up-regulated and 327 down-regulated metabolites.Flavonoids phloderin was up-regulated 140.55 times,and phosphatidylserine(PS)in glycerol and phospholipid metabolism was down-regulated 47.53 times.The differential metabolites are involved in various pathways such as glycolipid metabolism,amino acid biosynthesis and degradation,galactose metabolism,pyruvate and ketone metabolisms.CmSnf1 overexpression strains have advantages in ketone metabolism as compared with wild-type strains,suggesting that CmSnf1 gene plays an important role in the pharmacological effects of Cordyceps militaris.

关 键 词：SNF1 蛹虫草 Cmsnf1基因过表达 代谢组学 

分 类 号：S567.3[农业科学—中草药栽培]