冠突散囊菌发酵甘薯茶对高脂血症大鼠脂质代谢和肠道菌群紊乱的调节  

Effect of sweet potato tea fermented by Eurotium cristatum on lipid metabolism and gut microbiota in hyperlipidemic rats

作　　者：寇静 史琳娜 马添翼 KOU Jing;SHI Linna;MA Tianyi(School of Medical Technology,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学医学技术学院,陕西咸阳712046

出　　处：《食品与发酵工业》2023年第16期231-239,共9页Food and Fermentation Industries

基　　金：陕西省科技厅自然基础研究计划项目(2021JQ-743)。

摘　　要：探讨冠突散囊菌发酵甘薯茶对高脂饲料诱发的高脂血症大鼠脂质代谢和肠道菌群的影响。以健康雄性SD大鼠为试验对象,分为正常对照组、高脂对照组、茶饮高剂量和茶饮低剂量干预组,干预饲养8周。实验期间测量大鼠体重和脂质代谢相关指标,并收集肝脏及粪便进行病理学和肠道菌群分析。结果表明,发酵后甘薯茶能够显著降低高脂饮食大鼠血清总胆固醇、甘油三酯和低密度脂蛋白含量,促进肝脏脂质代谢,减轻脂肪在高脂饮食大鼠肝脏内堆积。测序结果显示,与模型组相比,肠道细菌中厚壁菌门与拟杆菌门的比例被提升,瘤胃球菌NK4A214 group、Christensenellaceae R-7 group菌属的丰度显著升高,Bacteroides等菌属的丰度显著降低。发酵后的甘薯茶能够有效改善高脂饮食大鼠的脂质代谢紊乱、肝损伤及肠道菌群紊乱。To investigate the influence of sweet potato tea fermented by Eurotium cristatum on lipid metabolism and gut microbiota in hyperlipidemia rats induced by high-fat diet.Healthy male SD rats as experimental subjects were divided into normal control group,high-fat control group,high-dose tea intervention group and low-dose tea intervention group for eight weeks.During the experiment,rats'body weight and serum biochemical indicators were measured,and the liver and feces were collected for pathological analysis and Intestinal microbiota detection after dissection.The results showed that the intervention of fermented sweet potato tea could significantly reduce the contents of serum total cholesterol,triglyceride and low-density lipoprotein,promote liver lipid metabolism and reduce the accumulation of fat in the liver of high-fat diet rats.The sequencing results showed that fermented sweet potato tea also markedly reversed gut dysbiosis in hyperlipidemic rats caused by high fat diet,as indicated by the significantly increased Firmicutes-to-Bacteroidetes ratios and the abundance of Ruminococcus_NK4A214 group and Christensenellaceae R-7 group,together with the decreased Bacteroides.In conclusion,fermented sweet potato tea could effectively improve the lipid metabolism disorder,liver injury and intestinal gut disorder in high-fat diet rats.

关 键 词：甘薯茶饮 发酵 高脂血症 肠道菌群 菌群分析 

分 类 号：R589.2[医药卫生—内分泌] TS272[医药卫生—内科学]