花椒中代森联·吡唑醚菌酯的残留分析及膳食风险评估  

Residue analysis and dietary risk assessment of metiramin and pyraclosrtrobin in Zanthoxylum bungeanum Maxim

作　　者：张耀海[1,2,3,4,5] 赵其阳 王成秋[1,2,3,4,5] 焦必宁 ZHANG Yaohai;ZHAO Qiyang;WANG Chengqiu;JIAO Bining(Citrus Research Institute,Southwest University&Chinese Academy of Agricultural Sciences,Chongqing 400712,China;Quality Supervision and Testing Center for Citrus and Seedling,Ministry of Agriculture and Rural Affairs,Chongqing 400712,China;Key Laboratory of Quality and Safety Control of Citrus Fruits,Ministry of Agriculture and Rural Affairs,Chongqing 400712,China;Laboratory of Quality and Safety Risk Assessment for Citrus Products,Ministry of Agriculture and Rural Affairs,Chongqing 400712,China;National Citrus Engineering Research Center,Chongqing 400712,China)

机构地区：[1]西南大学/中国农业科学院柑桔研究所,重庆400712 [2]农业农村部柑桔及苗木质量监督检测测试中心,重庆400712 [3]农业农村部柑橘类果品质量安全控制重点实验室,重庆400712 [4]农业农村部柑桔产品质量安全风险评估实验室,重庆400712 [5]国家柑桔工程技术研究中心,重庆400712

出　　处：《食品与发酵工业》2023年第16期261-268,共8页Food and Fermentation Industries

基　　金：重庆市技术创新与应用发展专项面上项目(cstc2021jscx-lyjsAX0007);农业农村部现代农业(柑橘)产业技术体系建设专项(CARS-26);国家重点研发计划项目(2019YFC1605604)。

摘　　要：该试验以花椒为研究对象,采用超高效液相色谱-串联质谱法和气相色谱-火焰光度检测器检测了代森联和吡唑醚菌酯及其代谢物乙撑硫脲在花椒生长发育过程中的残留量,研究了残留消解与转化规律,并探讨其可能产生的膳食健康风险。结果表明,建立的方法灵敏度及准确度良好,回收率为81%~107%,相对标准偏差为1.0%~9.5%,代森联、乙撑硫脲和吡唑醚菌酯的定量限均为0.05 mg/kg,能满足花椒中残留检测要求。按照良好农业规范在花椒上施用代森联和吡唑醚菌酯后,随着生长发育,代森联和吡唑醚菌酯均快速消解,施药28 d时鲜花椒消解率分别为95.3%~97.7%和94.1%~96.9%。鲜花椒加工成干花椒,能显著降低代森联在干花椒中的残留,而吡唑醚菌酯在干花椒中发生了显著的富集效应。花椒中代森联和吡唑醚菌酯急性和慢性膳食暴露风险均在可接受范围内。此研究结果可为我国花椒质量安全生产、监测和消费引导提供技术支撑和参考。In this experiment,Zanthoxylum bungeanum was used as the research object.The residues of metiramin,pyraclosrtrobin and its metabolite ethylene thiourea(ETU) in Zanthoxylum bungeanum during the growth and development were detected by ultra-high performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS) and gas chromatography coupled with flame photometric detector(GC-FPD).Results showed that the sensitivity and accuracy of the established method were good,the mean recoveries were 81%-107%,the relative standard deviations(RSDs) were 1.0%-9.5%,and the limits of quantitation(LOQs) were 0.05 mg/kg,which could meet the requirements of residue detection in Zanthoxylum bungeanum.According to good agricultural practices,after the application of metiramin and pyraclosrtrobin on Zanthoxylum bungeanum,with the growth and development,both metiramin and pyraclosrtrobi were rapidly digested,and the digestibility rates of fresh Zanthoxylum bungeanum were 95.3%-97.7% and 94.1%-96.9%,respectively after 28 days of application.Processing fresh Zanthoxylum bungeanum into dry could significantly reduce the residue of metiramin in dry Zanthoxylum bungeanum,while pyraclosrtrobin had a significant enrichment effect in dry Zanthoxylum bungeanum.The residual levels of metiramin and pyraclosrtrobin in Zanthoxylum bungeanum were within the acceptable range of acute and chronic dietary exposure risks to human health.The research results provided technical support and reference for the quality and safety production,monitoring and consumption guidance of Zanthoxylum bungeanum in China.

关 键 词：花椒 代森联 吡唑醚菌酯 残留分析 膳食风险 

分 类 号：S481.8[农业科学—农药学]