沉默信息调节因子2、苯并咪唑出芽抑制解除同源物蛋白-1在结直肠癌组织的表达及其临床意义  

Expression and clinical value of silent information regulator 2 and budding uninhibited by benzimidazoles-1in colorectal cancertissues

作　　者：徐延钧[1] 马良[2] 杨苏敏 韩超[1] 宋应明[1] Xu Yanjun;Ma Liang;Yang Sumin;Han Chao;Song Yingming(Department of Gastrointestinal Surgery,Heping Hospital Affiliated to Changzhi Medical College,Changzhi 046000,China;Department of Medical Laboratory,Heping Hospital Affiliated to Changzhi Medical College,Changzhi 046000,China;Department of Pathology,Heping Hospital Affiliated to Changzhi Medical College,Changzhi 046000,China)

机构地区：[1]长治医学院附属和平医院胃肠外科,长治046000 [2]长治医学院附属和平医院检验科,长治046000 [3]长治医学院附属和平医院病理科,长治046000

出　　处：《中华实验外科杂志》2023年第7期1390-1393,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨沉默信息调节因子2(SIRT2)和苯并咪唑出芽抑制解除同源物蛋白-1(BUB1)在结直肠癌组织中的表达水平及其临床价值。方法以2020年1月至2022年12月长治医学院附属和平医院诊治的120例结直肠癌患者癌组织和相匹配的肿瘤边缘>5 cm的癌旁组织标本作为研究对象, 采用免疫组织化学法检测SIRT2和BUB1在上述组织的表达, 收集患者临床资料, 应用χ^(2)检验分析两者的表达水平与结直肠癌患者病理特征和预后的关系。结果 SIRT2在结直肠癌组织中阳性表达率为41.67%(50/120), 明显低于癌旁组织中阳性表达率为80.83%(97/120), 两者差异有统计学意义(χ^(2)=38.78, P<0.01)。SIRT2表达水平与结直肠癌患者组织学分化程度、淋巴结转移、TNM分期明显相关(χ^(2)=5.481、6.607、4.631, P<0.05), SIRT2表达水平与结直肠癌患者性别、浸润深度、年龄、肿瘤部位无明显相关(χ^(2)=0.001、0.004、0.089、0.571, P>0.05)。BUB1在结直肠癌组织中阳性表达率为71.67%(86/120), 明显高于癌旁组织中阳性表达率为21.67%(26/120), 两者比较差异有统计学意义(χ^(2)=60.268, P<0.01)。BUB1表达水平与结直肠癌患者TNM分期、浸润深度、淋巴结转移明显相关(χ^(2)=5.363、7.164、6.225, P<0.05), BUB1表达水平与结直肠癌患者年龄、组织学分化程度、肿瘤部位、性别无明显相关(χ^(2)=0.161、0.031、0.060、0.571, P>0.05)。结论结直肠癌组织中SIRT2低表达、BUB1高表达, SIRT2和BUB1有助于判断结直肠癌恶性程度和预后。Objective To study the expression of silent information regulator 2(SIRT2)and bud-ding uninhibited by benzimidazoles-1(BUB1)in colorectal cancer tissues and their clinical value.Methods Totally,120 cases of colorectal cancer tissues and matched tumor margins>5 cm of paracancerous tissue specimens diagnosed and treated in Heping Hospital Affiliated to Changzhi Medical College from January 2020 to December 2022 were collected.The expression of SIRT2 and BUB1 in the above tissues was detec-ted by immunohistochemistry,and the clinical data of the patients were collected.The relationship between the expression levels of SIRT2,BUB1 and the pathological characteristics and prognosis of patients with colorectal cancer was analyzed by Chi-square test.Results The positive expression rate of SIRT2 in color-ectal cancer tissues was 41.67%(50/120),which was significantly lower than that in adjacent normal colorectal tissues[80.83%(97/120),χ^(2)=38.78,P<0.01].The expression level of SIRT2 was signifi-cantly correlated with histological differentiation,lymph node metastasis and tumor node metastasis(TNM)stage of colorectal cancer patients(respectively,χ^(2)=5.481,6.607,4.631,P<0.05),but not signifi-cantly with gender,depth of invasion,age and tumor location of colorectal cancer patients(respectively,χ^(2)=0.001,0.004,0.089,0.571,P>0.05).The positive expression rate of BUBI in colorectal cancer tissues was 71.67%(86/120),which was significantly higher than that in adjacent normal colorectal tissues[21.67%(26/120)],and the difference was statistically significant(χ^(2)=60.268,P<0.01).The expression level of BUB1 was significantly correlated with TNM stage,depth of invasion and lymph node metastasis in patients with colorectal cancer(respectively,χ^(2)=5.363,7.164,6.225,P<0.05),but not significantly with age,histological differentiation,tumor location and gender of colorectal cancer patients(respectively,χ^(2)=0.161,0.031,0.060,0.571,P>0.05).Conclusion SIRT2 is down-regulated and BUB1 is up-regulated in colorectal cancer tis

关 键 词：沉默信息调节因子2 结直肠癌 苯并咪唑出芽抑制解除同源物蛋白-1 免疫组织化学方法 

分 类 号：R735.34[医药卫生—肿瘤]