miRNA-34a靶向HDAC1对胃癌细胞增殖、侵袭和上皮间充质转化的影响  

MiRNA-34a inhibits the proliferation,invasion and epithelial-mesenchymal transition of gastric cancer cells by targeting and inhibiting HDAC1

作　　者：黄铄 梁志波 李兴杰 HUANG Shuo;LIANG Zhi-bo;LI Xing-jie(Department of General Surgery,Beijing Chaoyang Integrative Medicine Emergency Medical Center,Beijing 100021,China;Department of General Surgery,Affiliated Hospital of Hebei University,Baoding071000,China)

机构地区：[1]北京朝阳中西医结合急诊抢救中心普通外科,北京100021 [2]河北大学附属医院普通外科,河北保定071000

出　　处：《中国现代普通外科进展》2023年第8期605-611,共7页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨miR-34a通过靶向组蛋白去乙酰化酶1(HDAC1)对胃癌细胞增殖、侵袭和上皮间充质转化(EMT)的影响。方法:以胃癌组织及癌旁组织、体外培养的胃癌细胞系BGC-823、MKN28、AGS、SGC-7901以及人正常胃黏膜上皮细胞NGEC为研究对象。利用Lipofectamine 2000转染试剂对SGC-7901细胞进行转染,并分组为空白对照组、miR-NC组、miR-34a mimics组、miR-34a mimics+pcDNA组、miR-34a mimics+pc-HDAC1组。结果:与癌旁组织和人正常胃黏膜上皮细胞NGEC相比,胃癌组织和细胞系中miR-34a表达降低(P<0.05),HDAC1 mRNA表达升高(P<0.05)。miR-34a mimics可提高miR-34a表达、E-cadherin表达上调,细胞增殖活性、PCAN、Ki-67、MMP-2、MMP-9、N-cadherin、Vimentin蛋白表达降低,细胞侵袭数明显减少(P<0.05)。miR-34a靶向负调控HDAC1蛋白表达(P<0.05)。pc-HDAC1可使miR-34a mimics对SGC-7901细胞增殖、侵袭、EMT的抑制作用得到逆转(P<0.05)。结论:miR-34a过表达可能通过靶向负调控HDAC1表达抑制胃癌细胞增殖、侵袭和EMT。Objective:To investigate the effects of miR-34a on the proliferation,invasion and epithelial-mesenchymal transition(EMT)of gastric cancer cells by targeting histone deacetylase 1(HDAC1).Methods:The gastric cancer tissues and adjacent tissues collected in our hospital,gastric cancer cell lines BGC-823,MKN28,AGS,SGC-7901 and human normal gastric mucosal epithelial cells NGEC cultured in vitro were used as the research objects.SGC-7901 cells were transfected with Lipofectamine 2000 transfection reagent and grouped into blank control group,miR-NC group,miR-34a mimics group,miR-34a mimics+pcDNA group,and miR-34a mimics+pc-HDAC1 group.Results:Compared with adjacent tissues and human normal gastric mucosal epithelial cells NGEC,the relative expression of miR-34a in gastric cancer tissues and cell lines were reduced(P<0.05),the relative expression of HDAC1 mRNA was increased(P<0.05).After transfection of miR-34a mimics,the relative expression of miR-34a and E-cadherin were up-regulated in SGC-7901 cells,the cell proliferation activity,the expression levels of PCAN,Ki-67,MMP-2,MMP-9,N-cadherin and Vimentin were decreased,the number of cell invasion was reduced(P<0.05).MiR-34a targeted and negatively regulated the expression of HDAC1 protein(P<0.05).pc-HDAC1 transfection could reverse the inhibitory effects of transfection of miR-34a mimics on the proliferation,invasion and EMT of SGC-7901 cells(P<0.05).Conclusion:The overexpression of miR-34a may inhibit the proliferation,invasion and EMT of gastric cancer cells by targeting and negatively regulating the expression of HDAC1.

关 键 词：胃癌 MIR-34A 组蛋白去乙酰化酶1 增殖 侵袭 上皮间充质转化 

分 类 号：R735.2[医药卫生—肿瘤]