家兔肌肉组织中保守miRNA的鉴定及进化分析  

Identification and Evolutionary Analysis of the Conserved miRNAs in the Muscle Tissue of Rabbits

作　　者：张翔宇[1] 张翠霞[1] 张凯[2] 袁定胜[1] 李钰莹 邝良德[1] 李丛艳[1] 郑洁[1] 任永军[1] 郭志强[1] 唐丽[1] 季杨[1] 杨锐[1] 雷岷[1] 黄邓萍[1] 谢晓红[1] 杨超[1] ZHANG Xiangyu;ZHANG Cuixia;ZHANG Kai;YUAN Dingsheng;LI Yuying;KUANG Liangde;LI Congyan;ZHENG Jie;REN Yongjun;GUO Zhiqiang;TANG Li;JI Yang;YANG Rui;LEI Min;HUANG Dengping;XIE Xiaohong;YANG Chao(Sichuan Animal Science Academy/Key Laboratory of Animal Genetics and Breeding in Sichuan Provine,Chengdu 610066,China;Sichuan Academy of Grassland Sciences,Chengdu 611743,China)

机构地区：[1]四川省畜牧科学研究院/动物遗传育种四川省重点实验室,成都610066 [2]四川省草原科学研究院,成都611743

出　　处：《四川农业大学学报》2023年第4期713-718,共6页Journal of Sichuan Agricultural University

基　　金：科研院所科技成果转化项目(2021JDZH0019);四川省畜牧科学研究院基本科研业务专项资金(SASA202105);四川省科技计划项目应用基础研究(2021YJ0267);四川省自然科学基金(2023NSFSC0171);农业部国家现代农业产业技术体系(CARS-43-D-1);四川省畜牧科学研究院基本科研业务专项资金(SASA202305)。

摘　　要：【目的】旨在鉴定家兔肌肉组织中的保守miRNA,获取其表达谱并分析其进化特征,进而为研究保守性miRNA在家兔生长发育过程中的生物学功能提供理论依据。【方法】采用Solexa技术对家兔背最长肌组织中miRNA进行了测序和分析。【结果】通过序列比对鉴定出521个保守的miRNA序列,分属于238个不同的家族。其中miR-1、miR-133、miR-206和miR-499等肌肉特异性表达的miRNA在肌肉中均有表达,且除miR-499外的其它3个miRNA在所测定样本中均高表达。10个动物保守性miRNA家族在S86专门化父系肌肉基因组中出现了丢失。靶基因显著富集于13条信号通路,其中Focal adhesion和PI3K-Akt信号通路与肌肉发育密切相关。【结论】研究鉴定的影响肌肉发育的miRNA及其靶基因富集通路,将有助于家兔生长调控机制的研究,加快可用于标记辅助选择的分子标记的开发。【Objective】Identification and evolutionary analysis of conserved miRNAs expression in rabbit muscle was studied,with the purposes of providing a theoretical basisfor the further studies of the biological functions of conserved miRNAs in rabbit growth and development activities.【Method】MiRNA libraries of table rabbit Longissimus muscle tissue were sequenced and analyzed via using Solexa technology.【Result】The results showed that 521 conserved miRNA sequences belonging to 238 different miRNA families were identified by sequence alignment among the obtained RNA pure sequences.miR-1,miR-133,miR-206 and miR-499 muscle-specific miRNAs were expressed in muscle.Except miR-499,the three other muscle-specific miRNAs were highly expressed in tables of rabbit muscle.10 animal-conserved miRNA families were lost in the muscle genomes of S86 specialized paternal lines.The target genes were significantly enriched in 13 signaling pathways,of which the Focal adhesion and PI3KAkt signaling pathways were closely associated with muscle development.【Conclusion】The identified and predicted miRNAs and their target gene enrichment pathways affecting muscle development will contribute to the study of growth regulatory mechanisms in table rabbit,which accelerates the development of molecular markers that can be used for marker assisted selection.

关 键 词：家兔 Solexa测序 微小RNA 生物信息学方法 

分 类 号：S829.1[农业科学—畜牧学]