DEPDC1在子宫内膜癌中表达上调  

DEPDC1 is up-regulated in uterine corpus endometrial cancer

作　　者：杨婷婷 黄洁 郭建新 韩健 YANG Tingting;HUANG Jie;GUO Jianxin;HAN Jian(Department of Obstetrics and Gynaecology,Army Medical Center of PLA(Daping Hospital),Army Medical University,Chongqing 400042,China)

机构地区：[1]陆军军医大学陆军特色医学中心妇产科,重庆400042

出　　处：《基础医学与临床》2023年第9期1399-1407,共9页Basic and Clinical Medicine

摘　　要：目的观察DEP结构域蛋白1(DEPDC1)在子宫内膜癌(UCEC)中的表达及作用。方法利用GEO和TCGA等公共数据库分析DEPDC1 mRNA在UCEC中的表达情况,以及其与临床病理参数及预后的相关性;利用组织芯片、免疫印迹和实时定量PCR检测DEPDC1在UCEC组织和细胞系中的表达;利用平板集落、CCK8法和裸鼠成瘤等实验检测DEPDC1对UCEC细胞系增殖的影响。结果DEPDC1 mRNA在UCEC中表达上调(P<0.001),其表达与UCEC临床分期(P<0.001)、组织分化程度(P<0.05)及病理类型(P<0.001)等临床病理参数显著相关;相较于DEPDC1 mRNA低表达的患者,高表达的患者疾病特异生存期(DSS)和无进展期(PFI)显著降低(P<0.01);DEPDC1在UCEC组织和细胞系中高表达,敲降DEPDC1后,UCEC细胞系的集落形成减少、细胞率降低(P<0.01)和体内成瘤能力下降(P<0.05)。结论DEPDC1在UCEC中表达上调,参与UCEC的恶性进展及影响患者预后,并在UCEC细胞系增殖中发挥重要调控作用。Objective To explore the expression and function of DEP domain containing 1(DEPDC1)in uterine corpus endometrial cancer(UCEC).Methods The Gene Expression Omnibus(GEO)and The Cancer Genome Atlas(TCGA)databases were used to analyze the expression of DEPDC1 mRNA in normal endometrial tissues and UCEC tissues,the relationship with patient clinicopathological parameters and prognosis.Immuno-histochemistry(IHC),Western blot and RT-qPCR were used to detect the expression of DEPDC1 in UCEC cell lines and tissue.The CCK-8 assay,colony formation assay,and tumor xenograft were used to investigate the effect of DEPDC1 on cancer proliferation.Results DEPDC1 mRNA was up-regulated in UCEC(P<0.001)and was correlated with the clinical stages(P<0.001),tissue differentiation(P<0.05)and histological type(P<0.001)of UCEC.Compared with the DEPDC1 mRNA low expression patients,the patients with high expression of DEPDC1 showed lower disease-specific survival(DSS)and progress-free interval(PFI)(P<0.01).The IHC,Western blot,and RT-qPCR showed that DEPDC1 was up-regulated both in UCEC tissue and cell lines.Down regulation of DEPDC1 inhibited the colony formation,invasion and tumor sphere formation,cell proliferation rate,and tumourigenesis in vivo.Conclusions DEPDC1 is up-regulated in UCEC that is correlated with the malignant progression and poor prognosis of UCEC.

关 键 词：子宫内膜癌 DEP结构域蛋白1(DEPDC1) 增殖 

分 类 号：R737.33[医药卫生—肿瘤]