微RNA-194在宫颈癌中的表达及临床意义  

Expression and clinical significance of miRNA-194 in cervical cancer

作　　者：崔桂荣[1] 张金华 刘亚宁[1] 李焱[1] CUI Guirong;ZHANG Jinhua;LIU Yaning;LI Yan(Department of Hematology,The First Hospital of Handan City,Handan,Hebei 056002,China)

机构地区：[1]邯郸市第一医院血液内科,河北邯郸056002

出　　处：《安徽医药》2023年第9期1857-1860,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨微RNA(miRNA)-194在宫颈癌组织中的表达情况及其临床意义。方法回顾性选取2013年5月至2016年1月于邯郸市第一医院行手术治疗的136例宫颈癌病人为研究对象,采用分层抽样选取58例宫颈癌病人的癌组织及相应癌旁正常组织,其余78例病人仅选取癌组织,获取136例宫颈癌组织标本及58例癌旁正常组织标本。采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测宫颈癌组织和癌旁组织中miRNA-194表达水平,分析miRNA-194表达与宫颈癌病人临床病理特征及预后的关系。结果qRT-PCR结果表明136例宫颈癌病人癌组织miRNA-194的表达量(1.140±0.655)明显低于癌旁组织(4.502±1.168)(P<0.001)。miRNA-194表达水平与宫颈癌病人的国际妇产科联盟(FIGO)分期(P=0.001)和肿瘤长径(P=0.033)相关。miRNA-194低表达的宫颈癌病人的生存期显著低于miRNA-194高表达病人[HR 95%CI=2.17(1.21,3.90),log-rank P=0.012]。Cox回归分析结果表明miRNA-194表达是影响宫颈癌病人预后的独立因素。结论miRNA-194在宫颈癌的发生发展中有一定作用,其在宫颈癌病人癌组织中更低,miRNA-194有可能成为宫颈癌诊断和预后的指标。Objective To detect the microRNA(miRNA)-194 expression level in cervical cancer,and to explore its clinical significance.Methods Totally 136 cervical cancer patients,who underwent surgery in The First Hospital of Handan City from May 2013 to January 2016,were retrospectively selected as research objects.Cancer tissue and corresponding adjacent normal cervical tissue specimens of 58 patients were collected by stratified sampling method and only cancer tissue were collected from the other 78 patients.Totally there were 136 specimens of cervical cancer tissue and 58 specimens of adjacent normal tissue.The expressions of miRNA-194 in cancer tissue and adjacent tissue were detected by quantitative real time polymerase chain reaction(qRT-PCR),and its relationship with clinicopathological features and prognosis of cervical cancer patients was analyzed.Results qRT-PCR results showed that the expression level of miRNA-194 in cancer tissue of 136 patients with cervical cancer was significantly lower than that in adjacent tissue[(1.140±0.655)vs.(4.502±1.168),P<0.001].The expression level of miRNA-194 in cervical cancer was associated with FIGO(International Federation of Gynecology and Obstetrics)stage(P=0.001)and tumor size(P=0.033).Overall survival of cervical cancer patients with lower levels of miRNA-194 was significantly shorter than those with higher levels of miRNA-194[HR 95%CI=2.17(1.21,3.90),log-rank P=0.012].Cox regression analysis results showed that miRNA-194 expression was an independent risk factor for prognosis of cervical cancer patients.Conclusion miRNA-194 plays a certain role in the occurrence and development of cervical cancer,the expression of which is lower in the cancer tissue of cervical cancer patients,and may serve as a potential diagnostic and prognostic marker for cervical cancer.

关 键 词：子宫肿瘤 子宫颈 微RNA-194 实时荧光定量逆转录聚合酶链反应 预后 

分 类 号：R737.33[医药卫生—肿瘤]