饲用大豆全株蛋白含量关联SSR标记筛选  

Screening of SSR markers associated with protein content in whole plant of forage soybean

作　　者：李忠旺 陈光荣[2] 刘新星 杨如萍[2] 朱天地 陈琛 陈子萱 陈玉梁 LI Zhongwang;CHEN Guangrong;LIU Xinxing;YANG Ruping;ZHU Tiandi;CHEN Chen;CHEN Zixuan;CHEN Yuliang(Institute of Biotechnology,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China;Institute of Dryland Agriculture,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730020 [2]甘肃省农业科学院旱地农业研究所,甘肃兰州730070

出　　处：《草业科学》2023年第8期2072-2081,共10页Pratacultural Science

基　　金：甘肃省农业科学院生物育种专项项目(2020GAAS11);甘肃省农业科学院重点研发项目(2021GAAS16、2022GAAS10);国家产业技术体系兰州大豆综合试验站建设项目(CARS04-CES17)。

摘　　要：提高全株蛋白含量是饲用大豆(Glycine max)最重要的育种目标。为筛选可用于饲用大豆全株高蛋白含量育种实践的有效分子标记,本研究以甘肃省农业科学院大豆育种课题组前期筛选的55份饲用大豆核心种质资源作为供试群体材料,以大豆鼓粒期全株蛋白含量为目标性状,利用国内外文献报道的40个蛋白含量相关SSR标记对受试群体遗传多样性、群体结构进行分析,通过一般混合线性模型(GLM)和混合线性模型(MLM)两种模型对大豆全株蛋白含量与标记进行关联分析。结果表明,从40个SSR标记中筛选出多态性高、重复性好的标记21个,利用这21个SSR标记在群体中共检测出118个等位变异,每个标记的等位基因数变幅为3~10个,平均为5.6个;利用遗传相似度UPGMA聚类分析可将55份材料分成I、II、III 3类,遗传结构分析可分成POP1、POP2、POP3、POP4共4个亚群,两种方法分类结果不尽相同但基本相似;两种模型同时检测到6个SSR标记与全株蛋白含量显著相关(P<0.05),可用于全株高蛋白含量饲草大豆分子标记辅助选择育种实践。Increasing the protein content of the whole plant is the most important breeding goal in forage soybean.To screen for effective molecular markers that can be used in the breeding of high protein content in forage soybean,55 core germplasm resources were selected as the tested materials in this study.Then,40 pairs of SSR markers related to protein content reported both at home and abroad were used to analyze the genetic diversity and population structure of the tested materials.The correlation between protein content and selected markers was analyzed in whole soybean plant with a GLM(general linear model)and MLM(mixed linear model)using Tassel 2.1 software.The results showed that 21 of the 40 SSR markers had high polymorphism and good repeatability,so were selected for further study.A total of 118 alleles were detected in the population of the 21 SSR markers,and the number of alleles(Na)varied from 3 to 10,with an average of 5.6.From the results of genetic similarity UPGMA cluster analysis,55 materials could be divided into groupsⅠ,Ⅱ,andⅢ.Four subgroups(POP1,POP2,POP3 and POP4)could be created according to genetic structure analysis using Structure 2.3.4 software.The classification results of the two methods were different but basically similar.Six SSR markers were significantly associated with the protein content of the whole plant,which can be used to breed for a high protein content via the molecular-marker-assisted selection in forage soybean.

关 键 词：大豆 鼓粒期 蛋白质含量 关联分析 群体结构 遗传相似度 

分 类 号：S548[农业科学—作物学] S565.1