金荞麦茎叶总黄酮提取方法优化及其抗氧化活性  

Optimization of extraction method and antioxidant activity of total flavonoids from stems and leaves of Fagopyrum dibotrys

作　　者：孙光 张蓉 刘凤丹 陈光吉 李世歌[2,3] 尚以顺 宋莉 班骞 钟理 龙忠富 王茜 李小冬[2,3] SUN Guang;ZHANG Rong;LIU Fengdan;CHEN Guangji;LI Shige;SHANG Yishun;SONG Li;BAN Qian;ZHONG Li;LONG Zhongfu;WANG Qian;LI Xiaodong(Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)/Guihzhou Key Lab of Agro-bioengineering/Institute of Agro-bioengineering/College of Life Sciences,Guizhou University,Guiyang 550025,Guizhou,China;Institute of Prataculture,Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou,China;Guizhou Dingxin Agriculture and Animal Husbandry Technology Co.,Ltd.,Guiyang 550006,Guizhou,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院/山地植物资源保护与保护种质创新教育部重点实验室/贵州省农业生物工程重点实验室,贵州贵阳550025 [2]贵州省农业科学院草业研究所,贵州贵阳550006 [3]贵州鼎芯农牧科技有限公司,贵州贵阳550006

出　　处：《草业科学》2023年第8期2162-2170,共9页Pratacultural Science

基　　金：贵州省科技支撑计划项目“蛋鸡抗热应激功能性饲料产品研究与示范”(黔科合支撑[2021]一般157号);贵州省科技支撑计划项目“植物源添加剂调控贵州黑山羊生长速率技术研究与示范”黔科合支撑[2021]一般152;黔农科院种质资源项目[2022]14号。

摘　　要：本研究采用超声波辅助双水相萃取技术对金荞麦(Fagopyrum dibotrys)中黄酮类化合物的提取方法进行优化,并对所提取的活性物质进行抗氧化特性研究。首先以硫酸铵质量分数、液料比、超声时间为试验因子开展多因子响应面试验,以提取物中总黄酮含量为评价指标,优化提取工艺。再以DPPH(1,1-二苯基-2-三硝基苯肼)、OH−(羟基)、O_(2)^(−)(超氧阴离子)自由基清除率为指标,对金荞麦提取物的抗氧化能力进行评价。结果显示,金荞麦总黄酮最优制备条件为硫酸铵质量分数15%、液料比38꞉1、超声时间47 min,提取到的总黄酮含量为78.52 mg·g^(−1)。金荞麦提取物对DPPH、OH^(−)、O_(2)^(−)自由基清除率的IC50值分别为0.1182、4.0710、25.3800 mg·mL^(−1)。In this study,ultrasonic-assisted aqueous two-phase extraction technology was used to optimize the extraction method of flavonoids from Fagopyrum dibotrys,and the antioxidant properties of the extracted active substances were studied.To optimize the extraction process,a multi-factor response surface test was conducted using ammonium sulfate mass fraction,liquid-to-solids ratio,and ultrasonic time as independent variables.The total flavonoid content in the extract served as the evaluating parameter.The antioxidant capacity of F.dibotrys extract was evaluated by DPPH,OH^(−),O_(2)^(−)free radical scavenging rate.The results showed that the optimal preparation conditions of total flavonoids from F.dibotrys were as follows:ammonium sulfate mass fraction 15%,liquid-solid ratio 38꞉1,ultrasonic time 47 min,and the total flavonoids content was 78.52 mg·g^(−1).The IC50 values of DPPH,OH^(−),O_(2)^(−)free radical scavenging rate of F.dibotrys extract were 0.1182,4.0710,25.3800 mg·mL^(−1),respectively.

关 键 词：金荞麦 黄酮类化合物 双水相 萃取 响应面法 抗氧化 

分 类 号：S816.7[农业科学—饲料科学]