新生大鼠海马神经元原代细胞培养方法改良及鉴定  

Improvement and Identification of Primary Cell Culture Method of Neonatal Rat Hippocampal Neurons

作　　者：岳宇娇 徐平 YUE Yu-jiao;XU Ping(Department of Neuro-rehabilitation,Affiliated Hospital of Zunyi Medical University,Zunyi 563000,Guizhou,China;Department of Neurological,Affiliated Hospital of Zunyi Medical University,Zunyi 563000,Guizhou,China)

机构地区：[1]遵义医科大学附属医院神经康复科,贵州遵义563000 [2]遵义医科大学附属医院神经内科,贵州遵义563000

出　　处：《医学信息》2023年第17期111-114,共4页Journal of Medical Information

基　　金：贵州省科技厅科技计划项目(编号:黔科合支撑[2019]2796号)。

摘　　要：目的探索一种稳定、经济、实用的SD新生鼠海马神经元的原代培养方法。方法选用出生24 h内的SD新生鼠,分离新生大鼠双侧海马组织制备海马组织单细胞悬液,采用DMEM/F12+2%B27培养体系维持培养,观察海马神经元形态变化,取培养5 d的海马神经元原代培养细胞,采用微管相关蛋白2(MAP2)免疫荧光染色鉴定神经元纯度。结果提取的海马神经元细胞存活率高,生长状态良好;MAP2免疫荧光鉴定神经元纯度达(95.20±1.50)%。结论本培养方法在保证海马神经元细胞纯度及细胞活性的同时,降低原代神经元培养成本,是一种稳定、经济、实用的海马神经元原代培养方法。Objective To explore a stable,economical and practical primary culture method of hippocampal neurons in SD neonatal rats.Methods SD neonatal rats within 24 hours after birth were selected.The bilateral hippocampal tissues of neonatal rats were isolated to prepare single cell suspension of hippocampal tissues.The DMEM/F12+2%B27 culture system was used to maintain culture.The morphological changes of hippocampal neurons were observed.The primary cultured cells of hippocampal neurons cultured for 5 days were taken,and the purity of neurons was identified by microtubule-associated protein 2(MAP2)immunofluorescence staining.Results The extracted hippocampal neuron cells had high survival rate and good growth state.The purity of neurons identified by MAP2 immunofluorescence was(95.20±1.50)%.Conclusion This method can not only ensure the purity and activity of hippocampal neurons,but also reduce the cost of primary neuron culture.It is a stable,economic and practical primary culture method of hippocampal neurons.

关 键 词：海马 神经元 原代 培养 微管相关蛋白2 

分 类 号：R741[医药卫生—神经病学与精神病学]