circ_0003221/miR-330-3p在骨肉瘤组织中的表达及对U2-OS增殖迁移的影响  

The expression of circ_0003221/miR-330-3p in osteosarcoma tissue and its effects on the proliferation and migration of U2-OS

作　　者：闫晓红[1] 方美花 王伟 张军[2] YAN Xiaohong;FANG Meihua;WANG Wei(Department of Oncology,Baoji Central Hospital,Shaanxi,Baoji 721008,China)

机构地区：[1]陕西省宝鸡市中心医院肿瘤内科,721008 [2]陕西省宝鸡市中心医院骨科,721008

出　　处：《河北医药》2023年第16期2427-2430,共4页Hebei Medical Journal

基　　金：陕西省重点研发计划项目(编号:2021SF-353)。

摘　　要：目的探讨circ_0003221在骨肉瘤组织中的表达及对U2-OS增殖迁移的影响和分子机制。方法选择37例骨肉瘤患者瘤组织及瘤旁组织,实时荧光定量PCR(RT-qPCR)检测circ_0003221和miR-330-3p的表达水平,Pearson进行相关性分析。双荧光素酶报告实验检测circ_0003221和miR-330-3p的靶向关系。将骨肉瘤细胞U2-OS分为si-NC组、si-circ_0003221组、miR-NC组、miR-330-3p mimic组、si-circ_0003221+anti-miR-NC组、si-circ_0003221+miR-330-3p Inhibitor组;MTT法检测细胞增殖抑制率;克隆形成实验检测细胞克隆形成数;Transwell检测细胞迁移数;划痕实验检测划痕愈合率。结果与瘤旁组织比较,骨肉瘤组织中circ_0003221表达水平升高,miR-330-3p表达水平降低(P<0.05);circ_0003221与miR-330-3p的表达呈负相关。circ_0003221靶向调控miR-330-3p。干扰circ_0003221或过表达miR-330-3p,U2-OS增殖抑制率升高,克隆形成数和迁移细胞数减少,划痕愈合率降低(P<0.05)。抑制miR-330-3p表达可逆转干扰circ_0003221对U2-OS细胞增殖和迁移的影响。结论circ_0003221再骨肉瘤组织中高表达,干扰circ_0003221可通过上调miR-330-3p抑制骨肉瘤细胞U2-OS增殖、迁移。Objective To investigate the expression of circ_0003221/miR-330-3p in osteosarcoma tissue and its effects on the proliferation and migration of U2-OS,and to explore its molecular mechanism.Methods A total of 37 cases of osteosarcoma tissues and paracancerous tissues were selected,and RT-qPCR was used to detect the expression levels of circ_0003221 and miR-330-3p,and Pearson correlation analysis was performed.The dual luciferase reporter experiment was used to analyze the correlation between circ_0003221 and miR-330-3p.The osteosarcoma cells U2-OS were divided into si-NC group,si-circ_0003221 group,miR-NC group,miR-330-3p mimic group,si-circ_0003221+anti-miR-NC group,si-circ_0003221+miR-330-3p Inhibitor group.MTT was used to detect cell proliferation inhibition rate;clone formation test was used to detect cell clone formation number;Transwell was used to detect cell migration number;scratch test was used to detect scratch healing rate.Results Compared with those in paracancerous tissues,the expression levels of circ_0003221 in osteosarcoma tissue were increased,however,the expression levels of miR-330-3p were significantly decreased(P<0.05).The expression levels of circ_0003221 were negatively correlated with those of miR-330-3p,and circ_0003221 targetedly regulated miR-330-3p.Interfering with circ_0003221 or overexpression of miR-330-3p could significantly increase the U2-OS proliferation inhibition rate,and decrease the number of colonies formed and the number of migrating cells as well as the scratch healing rate(P<0.05).Moreover to inhibit the expression of miR-330-3p could reverse the effects of interfering with circ_0003221 on the proliferation and migration of U2-OS cells.Conclusion The expression levels of circ_0003221 are increased in osteosarcoma tissues,and iInterfering with circ_0003221 can inhibit the proliferation and migration of osteosarcoma cells U2-OS by up-regulating the expression of miR-330-3p.

关 键 词：circ_0003221 miR-330-3p 骨肉瘤 增殖 迁移 

分 类 号：R738.3[医药卫生—肿瘤]