韭菜子皂苷的提取与抗疲劳作用  

Extracting Steroidal Saponins of Chinese Chive Seeds and Anti-fatigue Activities Atudying

作　　者：达热卓玛 徐一达 沈婧 DARE Zhuo-ma;XU Yi-da;SHEN Jing(Branch Deparment of Haixi Centers for Disease Control and Prevention,Haixi Mongol and Tibetan Autonomous Prefecture Qinghai 817000,China;College of Food Science,Jiangnan University,Wuxi Jiangsu 214122,China)

机构地区：[1]海西州疾病预防控制中心业务科,青海海西蒙古族藏族自治州817000 [2]江南大学食品学院,江苏无锡214122

出　　处：《中华养生保健》2023年第18期8-12,共5页CHINESE HEALTH CARE

摘　　要：目的韭菜子为原料,对乙醇提物进行甾体皂苷的鉴别,并确定提取优化条件,得到最佳提取条件,测定抗疲劳活性。方法韭菜子经50%乙醇提取,MCI色谱柱分离,通过显色反应:醋酐-浓硫酸反应、五氯化锑反应及三氯醋酸反应,确定70%乙醇洗脱液中是否含有甾体皂苷,并通过阳虚小鼠模型研究韭菜子甾体皂苷对小鼠力竭游泳时间和血乳酸的影响。结果韭菜子70%乙醇洗脱物含有甾体皂苷成分,在50%乙醇,60℃,料液比1:25条件下提取4 h,韭菜子总皂苷含量的均值为3.28%,为最佳提取条件,可显著延长阳虚小鼠游泳力竭时间,差异有统计学意义(P<0.05),降低血乳酸含量,差异有统计学意义(P<0.05)。结论韭菜子甾体皂苷提取物可改善小鼠运动疲劳的状态,具有显著抗疲劳活性。Objective To identify the steroidal saponins from Chinese chive seeds as raw material by ethanol,and to determine the extraction optimization conditions to obtain the best extraction conditions and determine the anti-fatigue activity.Methods Chinese chive seeds were extracted by 50%ethanol,separated by MCI column,and determined whether the 70%ethanol eluate contained steroidal saponins by color development reactions:Liebermann–Burchard(LB)colorimetric assay,Kahlenberg colorimetric assay,Rosen-Heimer colorimetric assay,and the eff ect of steroidal saponins of Chinese chive seeds on the exhaustive swimming time and blood lactic acidin(LAC)was studied by Yang defi ciency mouse model.Results The 70%ethanol eluate of Chinese chive seeds contained steroidal saponins,and the mean value of total saponin content of Chinese chive seeds was 3.28%under 50%ethanol,60℃,and 1:25 material to liquid ratio for 4 hours,which was the best extraction condition and could signifi cantly prolong exhaustive swimming time(P<0.05)and reduce blood lactic acid content in Yangdefi cient mice(P<0.05).Conclusion Chinese chive seed steroidal saponin extract can improve the state of exercise fatigue in mice and has signifi cant anti-fatigue activity.

关 键 词：韭菜子 甾体皂苷 提取 鉴定 抗疲劳 

分 类 号：R151.3[医药卫生—营养与食品卫生学]