转录因子NFIB在乳腺癌中对细胞生长增殖和侵袭的影响  

Effect of transcription factor NFIB on cell proliferation and invasion in breast cancer

作　　者：张文婷 路军[1] 蒋兴然 Zhang Wenting;Lu Jun;Jiang Xingran(Department of Pathology,Beijing Chao-Yang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]首都医科大学附属北京朝阳医院病理科,北京100020

出　　处：《中国医师杂志》2023年第8期1199-1204,1208,共7页Journal of Chinese Physician

摘　　要：目的探讨转录因子细胞核因子I B(NFIB)在乳腺癌中对细胞生长增殖和侵袭的影响。方法构建慢病毒pLKO.1-shNFIB质粒,包装并分别感染至人雌激素受体阳性(ER+)乳腺癌细胞系MCF-7和三阴乳腺癌(TNBC)细胞系MDA-MB-231,稳定敲低NFIB并通过Western blot法验证;细胞计数实验(CCK-8)和克隆形成实验探讨敲低NFIB对乳腺癌细胞生长增殖的影响;利用转移小室(Transwell)实验和Western blot法检测上皮间质转化蛋白标志物表达,探究敲低NFIB对三阴乳腺癌细胞侵袭能力的影响;利用Kaplan-Meier生存分析网页数据(http://kmplot.com/analysis/),探究NFIB对ER+乳腺癌和三阴乳腺癌患者预后的影响。结果在MCF-7和MDA-MB-231乳腺癌细胞中,敲低NFIB抑制细胞生长增殖;在三阴乳腺癌MDA-MB-231细胞中,敲低NFIB促进间质标志物Fibronectin表达并促进细胞侵袭;NFIB的表达越低,三阴乳腺癌患者的预后越差,而NFIB的表达对ER+乳腺癌患者预后无影响。结论敲低NFIB抑制MCF-7细胞增殖,NFIB表达水平与ER+乳腺癌患者预后不相关;敲低NFIB抑制MDA-MB-231细胞的增殖但促进其侵袭;NFIB的低表达与三阴乳腺癌患者较差的预后相关。Objective To investigate the effect of transcription factor nuclear factor IB(NFIB)on cell proliferation and invasion in breast cancer.Methods The lentivirus pLKO.1-shNFIB plasmid was constructed,packaged and infected with human estrogen receptor positive(ER+)breast cancer cell line MCF-7 and triple-negative breast cancer(TNBC)cell line MDA-MB-231,respectively,NFIB was stably knocked down and verified by Western blot method;Cell count test(CCK-8)and clone formation test were used to investigate the effect of knockdown NFIB on the growth and proliferation of breast cancer cells;The transwell experiment and Western blot method were performed to detect the expression of epithelial mesenchymal transition protein markers.The effect of knockdown NFIB on the invasive ability of triple-negative breast cancer cells was explored;Kaplan-Meier survival was used to analyze web data(http://kmplot.com/analysis/)to explore the effect of NFIB on the prognosis of ER+breast cancer and triple-negative breast cancer patients.Results In MCF-7 and MDA-MB-231 breast cancer cells,knocking down NFIB inhibited cell growth and proliferation;In triple-negative breast cancer MDA-MB-231 cells,knocking down NFIB promoted the expression of interstitial marker fibronectin and promoted cell invasion;The lower the expression of NFIB,the worse the prognosis of triple negative breast cancer patients,while the expression of NFIB had no effect on the prognosis of ER+breast cancer patients.Conclusions Knocking down NFIB inhibits the proliferation of MCF-7 cells,and the expression level of NFIB is not related to the prognosis of ER+breast cancer patients;Knocking down NFIB inhibits the proliferation of MDA-MB-231 cells but promotes their invasion;The low expression of NFIB is associated with the poor prognosis of triple-negative breast cancer patients.

关 键 词：三阴性乳腺癌 细胞系 肿瘤 核因子ⅠB 细胞增殖 细胞侵袭 

分 类 号：R737.9[医药卫生—肿瘤]