HBV-DNA、甾醇氧-酰基转移酶1表达及肝细胞癌分化程度的相关性  

Correlation between HBV-DNA levels,SOAT1 expression and differentiation of hepatocellular carcinoma

作　　者：郑晶[1,2] 魏尚 邓慧孜 ZHENG Jing;WEI Shang;DHENG Huizi(Department of Pathology,the First Affiliated Hospital of Hainan Medical University,Haikou,Hainan 571101,China;Department of Pathology,Hainan Medical University,Haikou,Hainan 571101,China)

机构地区：[1]海南医学院第一附属医院病理科,海南海口571101 [2]海南医学院病理教研室,海南海口571101

出　　处：《中国热带医学》2023年第7期710-714,共5页China Tropical Medicine

基　　金：海南省自然科学基金项目(No.820RC763)。

摘　　要：目的 探讨HBV-DNA水平、甾醇氧-酰基转移酶1(Sterol O-acyltransferase, SOAT1)表达及肝细胞癌肿瘤分化程度之间的相关性,为了解肝细胞癌发展提供科学依据。方法 收集58例HBV相关肝细胞癌病例及病历资料中HBV-DNA水平数据,同时收集病例癌组织及其配对癌旁组织,采用免疫组织化学方法检测SOAT1表达情况并评估肿瘤分化程度。结果 SOAT1高表达病例组和SOAT1低表达组HBV-DNA高水平率分别为81.1%(30/37)和19.0%(4/21),差异有统计学意义(χ^(2)=21.253,P<0.05),且SOAT1表达与HBV-DNA水平之间呈相关性(χ^(2)=7.021,P<0.05)。低分化肝细胞癌病例组中HBV-DNA高水平率为71.1%(27/38),中高分化肝细胞癌组中HBV-DNA高水平率为35.0%(7/20),差异具有统计学意义,且HBV-DNA水平与肿瘤分化程度之间呈现相关性(χ^(2)=7.021,P<0.05)。SOAT1在所有收集病例中整体高表达率为63.8%(37/58),在所有配对癌旁组织中均无表达(0/58),且SOAT1蛋白在癌组织中的表达水平与肿瘤分化程度呈现相关性(χ2=19.889,P<0.05)。在低分化病例组中SOAT1呈现高表达,高表达率为84.2%(32/38),在较高分化病例组的HCC样本中SOAT1普遍呈现低表达,高表达率仅为25.0%(5/20)。结论 HBV-DNA水平与肝细胞癌分化程度相关,低分化癌中HBV-DNA水平更高;SOAT1表达水平亦与肝细胞癌分化程度相关,低分化癌中SOAT1表达水平亦更高;且HBV-DNA水平与SOAT1表达水平呈正相关,而SOAT1是细胞脂质代谢的关键酶,提示HBV感染可能影响SOAT1的功能及水平从而干预肝细胞脂质代谢而参与肿瘤的发生及演进。Objective To investigate the correlation between HBV-DNA level,sterol O-acyltransferase(SOAT1)expression and tumor differentiation of hepatocellular carcinoma.Methods The clinical and HBV-DNA level data from 58 cases of HBV-associated hepatocellular carcinoma were collected,and the cancer tissues and their paired paracancerous tissues were collected to detect SOAT1 expression by immunohistochemistry and evaluate tumor differentiation.Correlation was statistically analyzed using chi-square tests.Results The high-level rate of HBV-DNA in the SOAT1 high expression group was 81.1%(30/37)compared to 19.1%(4/21)of the SOAT1 low expression group,with statistical significance,and there was also a correlation between SOAT1 expression and HBV-DNA levels(χ^(2)=21.253,P<0.05).In the low differentiation hepatocellular carcinoma group,the rate of HBV-DNA high levels was 71.1%(27/38),while it was 35.0%(7/20)in the wellmoderate differentiation group,with statistical significance.There was also a significant correlation between HBV-DNA levels and tumor differentiation degree(χ^(2)=7.021,P<0.05).The overall positive rate of SOAT1 expression in all collected cases was 63.8%(37/58),with no expression(0/58)detected in all paired paracancerous tissues,with statistical significance(P<0.05).Furthermore,the expression level of SOAT1 protein in cancer tissues was correlated with the degree of tumor differentiation(χ^(2)=19.889,P<0.05).SOAT1 was generally highly expressed in the low differentiated case group,with a positive rate of 84.2%(32/38),while SOAT1 was generally low expression or no expression in HCC samples with a higher degree of differentiation,with only a few samples exhibiting high expression,with a high expression rate of 25.0%(5/20).Conclusions There is a correlation between HBV-DNA levels and hepatocellular carcinoma differentiation degree,with higher levels of HBV-DNA detected in low differentiation tumors.Additionally,the expression level of SOAT1 is also related to the degree of differentiation of hepatocellular carci

关 键 词：肝细胞性肝癌 甾醇氧-酰基转移酶1 HBV-DNA 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤]