miR-381-3p在宫颈癌中靶基因及信号通路的分析  

Analysis of miR-381-3p as a Target Gene and Signaling Pathway in Cervical Cancer

作　　者：魏林珍[1] 马晓梅[1] 张郎 陈凡 秦天生[1] WEI Linzhen;MA Xiaomei;ZHANG Lang(Department of Obstetrics and Gynecology,Gansu Provincial Hospital,Lanzhou City,Gansu Province 730000;不详)

机构地区：[1]甘肃省人民医院妇产科,甘肃省兰州市730000 [2]兰州大学基础医学院 [3]甘肃中医药大学

出　　处：《医学理论与实践》2023年第17期2886-2889,共4页The Journal of Medical Theory and Practice

基　　金：甘肃省自然科学基金(21JR1RA012);兰州市人才创新项目(2020-RC-52);甘肃省人民医院在研培优项目(19SYPYB-21);甘肃省中医药管理局科研项目(GZKG-2021-52)。

摘　　要：目的:分析miR-381-3p在宫颈癌组织中的表达情况,并探索其可能的靶基因及分子调控机制,为宫颈癌的防治提供思路。方法:本研究通过TCGA数据库了解miR-381-3p在宫颈癌与宫颈癌旁组织中的表达,采用miRWalk、miRDB和miRTarBase获取下游靶基因,并用DAVID对下游靶基因行GO功能注释及KEGG富集分析,再构建蛋白与蛋白相互作用网络并找出关键基因,最后验证关键基因的表达。结果:成熟的miR-381-3p在多个物种间的序列有高度保守性,TCGA数据库显示:相比癌旁组织,宫颈癌组织中的miR-381-3p表达下调。GO功能注释参与RNA聚合酶Ⅱ启动子转录进行正向调节的生物学过程、细胞核等细胞组分、蛋白质结合等分子功能。KEGG表明靶基因富集于癌症通路、乳腺癌、胃癌、Hippo、MAPK等信号通路。ESR1、LEF1、PBX1、CCND2、FGFR2、CADM1、TCF3、MPP6、PVRL1、UBE3A是miR-381-3p的关键基因,还发现ESR1、PBX1、CCND2在宫颈癌的表达水平低于正常组织。结论:miR-381-3p在宫颈癌中呈低表达,其可能作为抑癌基因对宫颈癌的生物学过程起一定的作用,有望成为宫颈癌诊治的新靶点。Objective:To analyse the expression of miR-381-3p in cervical cancer tissues and explore its possible target genes and molecular regulatory mechanisms.This will provide ideas for the prevention and treatment of cervical cancer.Methods:This study analysed miR-381-3p expression in cervical cancer and paracancerous tissue using the TCGA database.MiRWalk,miRDB and miRTarBase were used to access the downstream target genes,DAVID was used to annotate the downstream target genes with GO and KEGG enrichment analysis.Next,the Protein-Protein Interaction Network was constructed,the key genes were identified,and finally the expression of the key genes was checked to see if the key genes were expressed.Results:TCGA database showed that expression of miR-381-3p was downregulated in cervical carcinoma compared with cervical adenocarcinoma.GO showed enrichment for biological processes such as positive regulation of transcription from the RNA polymeraseⅡpromoter,cellular components such as the nucleus,and molecular functions such as protein binding.KEGG signalling pathways are enriched in signalling pathways such as cancer pathways,breast cancer,gastric cancer,Hippo,MAPK,etc.The key genes for miR-381-3p are ESR1,LEF1,PBX1,CCND2,FGFR2,CADM1,TCF3,MPP6,PVRL1,and UBE3A.It was found that the expression of ESR1,PBX1 and CCND2 in cervical cancer was down-regulated compared with that in normal tissue.Conclusion:miR-381-3p is down-regulated in cervical cancer and may be involved in the biological process of cervical cancer as an oncogene,making it a potential new target for diagnosing and treating cervical cancer.

关 键 词：宫颈癌 miR-381-3p TCGA 靶基因 

分 类 号：R737.33[医药卫生—肿瘤]