原发性肝癌组织中层粘连蛋白3、黏着斑激酶蛋白表达及其与患者临床病理特征和预后关系研究  

Expression of LAMA3 and FAK proteins in primary liver cancer and their relationship with clinicopathological features and prognosis of patients

作　　者：刘立友 吴东洋 蔡青山 郑建兴 李树栋 刘东 LIU Liyou;WU Dongyang;CAI Qingshan;ZHENG Jianxing;LI Shudong;LIU Dong(Department of Hepatobiliary Surgery,Tangshan Central Hospital,Tangshan 063000,China)

机构地区：[1]唐山中心医院肝胆外科,河北唐山063000

出　　处：《陕西医学杂志》2023年第9期1259-1263,共5页Shaanxi Medical Journal

基　　金：河北省医学科学研究课题(20221840)。

摘　　要：目的:探讨层粘连蛋白3(LAMA3)、黏着斑激酶(FAK)蛋白在原发性肝癌组织中的表达情况,分析它们与患者临床病理特征和预后的关系。方法:选取接受手术治疗的原发性肝癌患者75例,收集癌组织和癌旁组织标本。采用免疫组化染色检测癌组织和癌旁组织LAMA3、FAK蛋白表达。术后随访肝癌患者3年,统计生存情况。分析肝癌组织LAMA3、FAK蛋白表达与患者临床病理特征的关系。采用Kaplan-Meier生存曲线分析LAMA3、FAK蛋白表达与原发性肝癌患者预后的关系。采用Logistic回归分析原发性肝癌患者预后的影响因素。结果:肝癌组织LAMA3和FAK蛋白阳性表达率高于癌旁组织LAMA3和FAK蛋白阳性表达率(均P<0.05)。有淋巴结转移和血管侵犯的患者肝癌组织LAMA3、FAK蛋白阳性表达率高于无淋巴结转移和无血管侵犯的患者(均P<0.05)。术后随访3年,生存患者35例,病死患者40例。Kaplan-Meier生存曲线分析显示,肝癌组织LAMA3、FAK蛋白表达阳性患者3年累积生存率低于LAMA3、FAK蛋白表达阴性患者(均P<0.05)。Logistic回归分析发现,有淋巴结转移、有血管侵犯、LAMA3蛋白阳性和FAK蛋白阳性是原发性肝癌患者术后3年预后的独立危险因素(均P<0.05)。结论:原发性肝癌组织中LAMA3、FAK蛋白呈高表达,且两者阳性表达与患者淋巴结转移、血管侵犯及预后有关,可能作为原发性肝癌潜在的预后标志物。Objective:To investigate the expression of laminin 3(LAMA3)and focal adhesion kinase(FAK)proteins in primary liver cancer tissues,and to analyze their relationship with clinicopathologic features and prognosis of patients.Methods:Seventy-five patients with primary liver cancer undergoing liver cancer resection were selected,and the cancer tissue and paracancerous tissue specimens were collected.Immunohistochemical staining was used to detect the expression of LAMA3 and FAK proteins in cancer tissues and paracancerous tissues.Patients were followed up for 3 years to calculate their survival status.The relationship between the expression of LAMA3,FAK proteins in cancer tissues and clinicopathological characteristics was analyzed.Kaplan-Meier survival curve was used to analyze the relationship between the expression of LAMA3,FAK proteins and the prognosis of patients with primary liver cancer.Logistic regression analysis was used to analyze the prognostic influencing factors of patients with primary liver cancer.Results:The positive expression rates of LAMA3 and FAK proteins in liver cancer tissues were higher than those in adjacent tissues(both P<0.05).The positive expression rates of LAMA3 and FAK proteins in liver cancer tissues of patients with lymph node metastasis and vascular invasion were higher than those of patients without lymph node metastasis and vascular invasion(both P<0.05).After 3 years of follow-up,35 patients survived and 40 patients died.Kaplan-Meier survival curve analysis showed that the 3-year cumulative survival rate of patients with positive LAMA3 and FAK proteins expression in liver cancer tissues was lower than that of patients with negative LAMA3 and FAK proteins expression(both P<0.05).Logistic regression analysis showed that lymph node metastasis,vascular invasion,LAMA3 protein positive and FAK protein positive were independent risk factors for the 3-year prognosis of patients with primary liver cancer(all P<0.05).Conclusion:LAMA3 and FAK proteins are highly expressed in primary liver c

关 键 词：原发性肝癌 层粘连蛋白3 黏着斑激酶 临床病理特征 淋巴结转移 血管侵犯 预后 

分 类 号：R735.7[医药卫生—肿瘤]