茶树细胞壁关联蛋白激酶基因家族的鉴定与表达分析  

Identification and Expression Analysis of the Wall-associated Kinase Gene Family in Camellia sinensis

作　　者：焦小雨 吴琼 刘丹丹 孙明慧 王文杰 JIAO Xiao-Yu;WU Qiong;LIU Dan-Dan;SUN Ming-Hui;WANG Wen-Jie(Tea Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230001,China)

机构地区：[1]安徽省农业科学院茶叶研究所,合肥230001

出　　处：《农业生物技术学报》2023年第9期1816-1831,共16页Journal of Agricultural Biotechnology

基　　金：安徽省重大专项(202003a06020021);安徽省茶叶产业技术体系(AHCYJSTX-11);安徽农业大学茶树生物学与资源利用国家重点实验室开放基金(SKLTOF20220120);安徽省农业科学院成果推广项目(2023YL031)。

摘　　要：细胞壁关联蛋白激酶(wall-associated kinase, WAK)是一类特殊的类受体蛋白激酶(receptor like kinase, RLK),在调节细胞生长和防御胁迫反应等方面发挥着重要作用。本研究利用生物信息学方法从茶树(Camellia sinensis)全基因组中鉴定出27个CsWAK成员,主要以基因簇的形式分布于2个染色体和1个组装片段上。根据系统进化关系将CsWAK分为5个亚组(Ⅰ~Ⅴ),同一亚组中的多数成员具有相似的基因结构和保守基序。转录组数据分析结果显示,大多数CsWAK成员在茶树的叶中高表达,其次是在根和茎中。在激素、低温、高盐等逆境胁迫下,CsWAK中多数成员基因表达变化差异不明显,少数基因表达变化显著,具有多样化和特异性。此外,对部分响应冷胁迫、PEG胁迫和盐胁迫的差异表达基因进行了qPCR分析。结果显示,低温处理下,所选9个基因均呈现先上升后下降的表达模式,且多数基因在低温处理3 h表达水平上调最为明显;在PEG处理下,所选9个基因的表达水平与转录组数据略有不同,但表达趋势相似;在NaCl处理下,所选9个基因的表达水平较未处理时显著下调,其中,CsWAK14对盐胁迫最为敏感。本研究结果为进一步解析CsWAK基因的生物学功能提供参考。The wall-associated kinase(WAK)is a unique class of receptor-like kinase(RLK),which plays an important role in regulating cell growth and defense against stress response.In this study,27 CsWAK genes were identified from the tea plant(Camellia sinensis)genome using bioinformatics methods,which were mainly clustered on 2 chromosomes and 1 contig.These CsWAK were classified into 5 subgroups(Ⅰ~Ⅴ)based on the phylogenetic analysis of the protein sequences,and most members of the same subgroup had similar gene structures and conserved motifs.The transcriptome data analysis showed that most CsWAK were expressed primarily in tea leaves,followed by roots and stems.Most members of the CsWAK family had little difference in response to biotic/abiotic stresses such as hormone,low temperature,and high salt.The difference of most CsWAK gene expression were not significant,only several CsWAK gene expression had significant difference,with diversity and specificity.In addition,some differentially expressed CsWAK in response to cold treatment,PEG treatment and salt treatment were analyzed by qPCR.The results showed that under the stress of low-temperature,the selected 9 genes all showed an expression pattern that rose first and then fell,and most of the genes were significantly upregulated at low-temperature treatment for 3 h;under the stress of PEG,the expression level of the selected 9 genes was slightly different from the transcriptome data,but the expression trend was similar;and under NaCl treatment,the expression level of the selected 9 genes was significantly downregulated compared to that of untreated,among them,CsWAK14 was the most sensitive to salt stress.These results provide a reference for further analysis of the biological functions of CsWAK gene.

关 键 词：茶树 细胞壁关联蛋白激酶(WAK)基因家族 胁迫应答 生物信息学 

分 类 号：S571.1[农业科学—茶叶生产加工]