Blue LED promotes the chemosensitivity of human hepatoma to Sorafenib by inducing DNA damage  

作　　者：TONG WANG JINHUAN HONG JIAJIE XIE QIAN LIU JINRUI YUE XUTING HE SHIYU GE TAO LI GUOXIN LIU BENZHI CAI LINQIANG LI YE YUAN 

机构地区：[1]Department of Pharmacy(The University Key Laboratory of Drug Research,Heilongjiang Province),The Second Affiliated Hospital of Harbin Medical University,Harbin,China [2]Department of General Surgery,The First Affiliated Hospital of Harbin Medical University,Harbin,China [3]National Key Laboratory of Frigid Cardiovascular Disease,Harbin,China [4]Department of Pharmacology(The State-Province Key Laboratories of Biomedicine Pharmaceutics of China,Key Laboratory of Cardiovascular Research,Ministry of Education),College of Pharmacy,Harbin Medical University,Harbin,China [5]Department of Clinical Pharmacology,College of Pharmacy,Harbin Medical University,Harbin,China

出　　处：《BIOCELL》2023年第8期1811-1820,共10页生物细胞（英文）

基　　金：supported by grants from the National Key Research and Development Program of China(2017YFB0403802);the Outstanding Youth Project of the Natural Science Foundation of Heilongjiang Province(YQ2020H019);the Huaier Fund in 2023 from Chen Xiao-Ping Foundation for the Development of Science and Technology of Hubei Province.

摘　　要：Background:Phototherapies based on sunlight,infrared,ultraviolet,visible,and laser-based treatments present advantages like high curative effects,small invasion,and negligible adverse reactions in cancer treatment.We aimed to explore the potential therapeutic effects of blue light emitting diode(LED)in human hepatoma cells and decipher the underlying cellular and molecular mechanisms.Methods:Wound healing and transwell assays were employed to probe the inhibition of the invasion and migration of hepatocellular carcinoma cells in the presence of blue LED.The sphere-forming test was used to evaluate the effect of LED blue light irradiation on cancer stem cell properties.Immunofluorescence and western blotting were used to detect the changes inγ-H2AX.The Cell Counting Kit-8 assay,5-ethynyl-2′-deoxyuridine staining,and colony formation assay were used to detect the combined effect of blue LED and sorafenib on cell proliferation inhibition.Results:We demonstrated that the irradiation of blue LED light in hepatoma cells could lead to cell proliferation reduction along with the increase of cell apoptosis.Simultaneously,blue LED irradiation also markedly suppressed the migration and invasion ability of human hepatoma cells.Sphere formation analysis further revealed the decreased cancer stemness of hepatoma cells upon blue LED irradiation.Mechanistically,blue LED irradiation significantly promoted the expression of the phosphorylation of the core histone protein H2AX(γ-H2AX),a sensitive molecular marker of DNA damage.In addition,we found that the combined treatment of blue LED irradiation and sorafenib increased cancer cell sensitivity to sorafenib.Conclusion:Collectively,we demonstrated that blue LED irradiation exhibited anti-tumor effects on liver cancer cells by inducing DNA damage and could enhance chemosensitivity of cancer cells,which represents a potential approach for human hepatoma treatment.

关 键 词：Blue LED Irradiation Liver cancer DNA damage Chemotherapy resistance 

分 类 号：R735.7[医药卫生—肿瘤]