鼠尾草酸调节CXCR7/CXCL12轴对胃癌AGS细胞增殖、迁移和侵袭能力的影响  

Carnosic acid affects the proliferation,migration,and invasion of gastric cancer AGS cells by regulating CXCR7/CXCL12 axis

作　　者：张鑫 李迪诺 田蕾 朱金朋 韩向东 ZHANG Xin;LI Dinuo;TIAN Lei;ZHU Jinpeng;HAN Xiangdong(Department of Digestive Medicine,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning,China;Department of Gastric Surgery,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning,China)

机构地区：[1]锦州医科大学附属第一医院消化内科,辽宁锦州121000 [2]锦州医科大学附属第一医院胃外科,辽宁锦州121000

出　　处：《中国肿瘤生物治疗杂志》2023年第8期695-700,共6页Chinese Journal of Cancer Biotherapy

摘　　要：目的:探讨鼠尾草酸(CA)通过调节CXC基序趋化因子受体7(CXCR7)/CXC基序趋化因子配体(CXCL12)轴对胃癌AGS细胞增殖、迁移和侵袭的影响。方法:用不同浓度(0、5、10、20、40、80μg/mL))的CA处理胃癌AGS细胞,采用CCK-8法筛选合适的CA浓度;将AGS细胞分为对照组(未经处理的AGS细胞)、CA组(20μg/mL CA处理)、CA+siCXCR7组(转染siCXCR7+20μg/mL CA处理)、CA+siNC组(转染siNC+20μg/mL CA处理)、CA+vectorNC组(转染vectorNC+20μg/mL CA处理)、CA+vectorCXCR7组(转染vectorCXCR7+20μg/mL CA处理),采用CCK-8法检测AGS细胞增殖的变化,qPCR法检测细胞中CXCR7、CXCL12 mRNA表达水平的变化,Transwell实验检测细胞侵袭能力的变化,划痕实验检测细胞迁移能力的变化,WB法检测周期蛋白D1、Bcl-2、CXCR7、CXCL12、MMP-2蛋白表达的变化。结果:不同浓度CA均可抑制AGS细胞存活率,且浓度为20μg/mL时,细胞存活率接近50%,故选择20μg/mL CA用于后续研究。与对照组相比,CA组增殖率、侵袭数、迁移率、周期蛋白D1、MMP-2、Bcl-2、CXCR7、CXCL12 mRNA及蛋白表达显著降低(均P<0.05);与CA+siNC组相比,CA+siCXCR7组增殖率、侵袭数、迁移率、周期蛋白D1、MMP-2、Bcl-2、CXCR7、CXCL12 mRNA及蛋白表达显著降低(均P<0.05);与CA+vectorNC组相比,CA+vectorCXCR7组增殖率、侵袭数、迁移率、周期蛋白D1、MMP-2、Bcl-2、CXCR7、CXCL12 mRNA及蛋白表达显著增加(均P<0.05)。结论:CA可抑制AGS细胞增殖、迁移和侵袭,其机制可能与抑制CXCR7/CXCL12轴有关。Objective:To investigate the effects of carnosic acid(CA)on the proliferation,migration,and invasion of gastric cancer cells by regulating CXC chemokine receptor 7(CXCR7)/CXC chemokine ligand 12(CXCL12)axis.Methods:CCK-8 method was used to select appropriate concentrations of CA.Gastric cancer AGS cells were treated with CA at different concentrations(0,5,10,20,40,80μg/mL).AGS cells were divided into the control group(untreated AGS cells),CA group(20μg/mL CA treatment),CA+siCXCR7 group(siCXCR7 transfection+20μg/mL CA treatment),CA+siNC group(siNC transfection+20μg/mL CA treatment),CA+vectorNC group(vectorNC transfection+20μg/mL CA treatment),and CA+vectorCXCR7 group(vectorCXCR7 transfection+20μg/mL CA treatment).The proliferation of AGS cells was detected by CCK-8 method;the expression levels of CXCR7 and CXCL12 mRNA in cells were detected by qPCR;and the invasion of cells was detected by Transwell assay;the change of cell migration ability was detected by scratch assay,and the expressions of cyclin D1,Bcl-2,CXCR7,CXCL12 and MMP-2 were detected by WB assay.Results:Different concentrations of CA were all able to inhibit the survival rate of AGS cells.When the concentration was 20μg/mL,the survival rate of AGS cells was closed to 50%.20μg/mL CA was therefore chosen for subsequent research.Compared with the control group,the proliferation rate,invasion number,migration rate,cyclin D1,MMP-2,Bcl-2,CXCR7,CXCL12 mRNA and protein expression of the CA group decreased significantly(all P<0.05).Compared with the CA+siNC group,the proliferation rate,invasion number,migration rate,cyclin D1,MMP-2,Bcl-2,CXCR7,CXCL12 mRNA and protein expression in the CA+siCXCR7 group decreased significantly(all P<0.05).Compared with the CA+vectorNC group,the proliferation rate,invasion number,migration rate,cyclin D1,MMP-2,Bcl-2,CXCR7,CXCL12 mRNA and protein expression in the CA+vectorCXCR7 group increased significantly(all P<0.05).Conclusion:CA can inhibit the proliferation,migration,and invasion of AGS cells,which may be related to t

关 键 词：鼠尾草酸 CXCR7/CXCL12 胃癌 AGS细胞 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤] R730.54[医药卫生—临床医学]