组合性抗体在分流ASC-US病变中的应用价值  

Clinical Value of Combined Antibody in Shunt ASC-US Lesions

作　　者：王侠[1] 杨翠[1] WANG Xia;YANG Cui(West Anhui Health Vocational College,Lu’an Anhui 237000,China)

机构地区：[1]皖西卫生职业学院,安徽六安237000

出　　处：《湖北科技学院学报（医学版）》2023年第4期303-306,312,共5页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：2019年安徽省教育厅高校科学研究自然科学重点项目(KJ2019A1257)。

摘　　要：目的探讨组合性抗体在分流非典型磷状细胞(ASC-US)病变中的临床应用价值。方法选择本院接受宫颈脱落细胞学检查的240例ASC-US患者,经HR-HPV与TCT检测将患者分为低级别鳞状上皮内病变(LSIL)组与高级别鳞状上皮内病变(HSIL)组,采用免疫组化法检测联合抗体P16/SOX-2、P16/Ki67,分别进行HPV E6/E7 mRNA检测,所有病例行阴道镜宫颈活检,比较组合性抗体检测敏感度及特异度。结果LSIL组和HSIL组患者组织中SOX-2/P16联合表达阳性率均显著高于组内SOX-2表达、P16表达阳性率(P<0.05)。LSIL组和HSIL组患者组织中P16/Ki-67联合阳性率均显著高于组内P16、Ki-67阳性率(P<0.05)。LSIL组HPV E6/E7阳性率均低于组内P16/Ki-67阳性率(P<0.05),HSIL组患者组织中HPV E6/E7阳性率均低于组内SOX-2/P16和P16/Ki-67阳性率(P<0.05)。SOX-2/P16诊断ASC-US敏感度为92.2%,特异度为55.9%;SOX-2/P16诊断ASC-US敏感度为96.1%,特异度为29.4%。结论组合性抗体检测在ASC-US诊断就鉴别诊断中具有重要价值,可作为ASC-US患者分流的有效指标。Objective To explore the clinical application value of combined antibody in shunting ASC-US lesions.Methods A total of 240 ASC-US patients who underwent cervical exfoliative cytology examination in our hospital were selected and divided into low-grade squamous intraepithelial lesion(LSIL)group and high-grade squamous intraepithelial lesion(LSIL)group by the HRHPV and TCT detections.Combined antibody P16/SOX-2 and P16/Ki-67 were detected by immunohistochemical method.HPV E6/E7 mRNA were detected,respectively.All cases underwent colposcopy cervical biopsy to compare the combination antibody detection sensitivity and specificity.Results A total of 240 ASC-US patients who underwent cervical exfoliative cytology examination in our hospital were selected and divided into low-grade squamous intraepithelial lesion(LSIL)group and high-grade squamous intraepithelial lesion(LSIL)group by the HRHPV and TCT detections.Combined antibody P16/SOX-2 and P16/Ki-67 were detected by immunohistochemical method.HPV E6/E7 mRNA were detected,respectively.All cases underwent colposcopy cervical biopsy to compare the combination antibody detection sensitivity and specificity.Conclusion Combined antibody detection is of great value in diagnosing and differential diagnosing ASC-US,and can be used as an effective indicator to shunt the patients with ASC-US.

关 键 词：组合性抗体 分流 ASC-US病变 应用价值 

分 类 号：R737.77[医药卫生—肿瘤]