ARTP诱变选育环孢菌素A高产菌株及发酵工艺优化  

Study on Screening of High-Yield Cyclosporin A Producing Strain by Atmospheric and Room Temperature Plasma Mutagenesis and Optimization of Fermentation Conditions

作　　者：林仙菊 张祝兰[1,2] 严凌斌 陈洲琴[1,2] 杨煌建 温耀明 连云阳[1,2] LIN Xianju;ZHANG Zhulan;YAN Lingbin;CHEN Zhouqin;YANG Huangjian;WEN Yaoming;LIAN Yunyang(Fujian Provincial Key Laboratory of Screening for Novel Microbial Products,Fuzhou 350007,China;Fujian Institute of Microbiology,Fuzhou 350007,China)

机构地区：[1]福建省微生物研究所,福建福州350007 [2]福建省新药(微生物)筛选重点实验室,福建福州350007

出　　处：《工业微生物》2023年第4期158-163,共6页Industrial Microbiology

基　　金：中央引导地方科技发展专项(2017L3004);福建省公益类项目(2023R1005002)。

摘　　要：采用常压室温等离子体技术(Atmospheric Room Temperature Plasma,ARTP)对雪白白僵菌FIM-1809菌株进行诱变,得到一株遗传稳定性较高的突变高产菌FIM-1809-7,其产环孢菌素A能力较初始菌株提高约39%。通过单因素和正交设计试验优化,确定最佳发酵培养基组分及培养条件为:玉米浆粉6%、可溶性淀粉12%、葡萄糖1.2%、蛋白胨2%,pH 5.6,菌种菌龄为72 h,接种量10%,装液量70 m L/500 m L,发酵时间8 d,最终突变菌株产环孢菌素A效价较出发菌种增加了约53%。结果表明,采用ARTP技术结合发酵条件优化能够有效提高雪白白僵菌产环孢菌素A的能力,为该菌株的工业化应用奠定了良好的基础。A high genetic stable mutant strain FIM-1809-7 was obtained by Atmospheric and Room Temperature Plasma(ARTP)mutation from the strain Beauveria nivea FIM-1809,the mutant strain production capacity of cyclosporin A was 39%higher than the initial strain.Through single factor and orthogonal design experiments,the optimal fermentation medium components and culture conditions were determined as follows:corn starch 6%,soluble starch 12%,glucose 1.2%,peptone 2%,pH 5.6,seed age 72 h,inoculation amount 10%,bottling capacity was 70 mL/500 mL,fermentation time 8 d.The titer of cyclosporin A produced by the final mutant strain increased by about 53%compared to the original strain.The results indicate that combining ARTP technology with fermentation conditions optimization can effectively improve the ability of Beauveria bassiana to produce cyclosporin A,laying a good foundation for the industrial application of this strain,

关 键 词：雪白白僵菌 环孢素A 常压室温等离子体诱变 发酵参数优化 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ465