超声引导下真空旋切和微波消融兔乳腺肿瘤的实验研究  

Experimental study on ultrasound-guided vacuum-assisted biopsy and microwave ablation of rabbit breast tumors

作　　者：冯煜然 韦琳 李星[1] 占金琴 朱梅[1] FENG Yuran;WEI Lin;LI Xing;ZHAN Jinqin;ZHU Mei(Department of Ultrasound,the First Affiliated Hospital of Kunming Medical University,Yunnan Kunming 650032,China)

机构地区：[1]昆明医科大学第一附属医院超声科,云南昆明650032

出　　处：《现代肿瘤医学》2023年第17期3191-3197,共7页Journal of Modern Oncology

基　　金：云南省卫生科技计划项目(编号:2017NS039);云南省医学领军人才项目(编号:L-2019032);云南省基础研究计划重点项目(编号:202201AS070077);云南省基础研究计划(编号:202201AY070001-079)。

摘　　要：目的:对比超声引导下真空旋切(vacuum-assisted biopsy,VAB)和微波消融(microwave ablation,MWA)兔乳腺肿瘤的疗效及术后局部恢复情况。方法:将72只新西兰大白兔VX2乳腺肿瘤模型随机分为两组,每组36只,分别行超声引导下VAB与MWA,术后第1、3、7、14、30、60天进行超声检查、大体观察、病理HE染色,并采用免疫组化检测CD31、VEGF、TGF-β进行量化,评价疗效及局部愈合情况。结果:30天后VAB组的治疗成功率为75.0%,MWA组成功率为83.3%。VAB组术后血肿发生率为13.9%,随着时间增长逐渐缩小;MWA组皮肤烫伤发生率为13.9%。两组治疗后第7天肉芽组织达高峰,随后均减少,逐渐上皮化,MWA组稍晚。术后14天、30天、60天愈合量化指标VAB组高于MWA组。术后3天,VAB组的早期愈合标记物CD31表达量更高;术后14天、30天,MWA组VEGF水平更高,TGF-β水平下降速度更慢。结论:VAB和MWA微创治疗兔VX2乳腺肿瘤均有较好疗效。VAB组早期愈合优于MWA组,瘢痕形成更少。Objective:To compare the effect and healing of vacuum-assisted biopsy(VAB)and microwave ablation(MWA)in the treatment of breast cancer in rabbit.Methods:This study built 72 VX2 breast cancer models in rabbits and the rabbits received operation with VAB and MWA randomly,which were 36 in each group.General observation,ultrasound and pathological examination were used for evaluate of the treatments at 1st day,3rd day,7th day,14th day,30th day,60th day after the VAB and MWA.Immunohistochemical detection in CD31,VEGF,TGF-βwas used to quantify and evaluate the efficacy and local healing.Results:The success rate of VAB after 30 days was 75.0%,and that of MWA was 83.3%.The incidence of postoperative hematoma in VAB was 13.9%,which would gradually reduce with time.The incidence of postoperative scald in MWA was 13.9%.The granulation tissue reached the peak at 7th day in both group.Then the granulation tissue decreased and had gradually epithelialization,but the time of epithelialization in MWA was later.Quantification indicated that VAB was higer than MWA at 14th day,30th day,60th day.The CD31 expression rate of VAB was higher at 3rd day.The expression level of VEGF in MWA was higher at 14th day and 30th day,and the expression of TGF-βdecreased more slowly in MWA.Conclusion:VAB and MWA are both effective and safe methods for VX2 breast cancer in rabbits.VAB may be better than MWA in early healing,and scar formation is not as obvious as MWA.

关 键 词：超声引导 真空旋切术 微波消融术 乳腺肿瘤 VX2肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]