淫羊藿素对人舌鳞癌细胞CAL-27增殖、凋亡、迁移及侵袭的影响  

Effects of icaritin on proliferation,apoptosis,migration and invasion of human tongue squamous cell CAL-27

作　　者：曹华娇 傅玲玲 冯红超 CAO Huajiao;FU Lingling;FENG Hongchao(School of Stomatology,Guizhou Medical University,Guiyang,Guizhou,550001;Department of Oral and Maxillofacial Surgery,Guiyang Stomatological Hospital in Guizhou Province,Guiyang,GuiZhou,550002)

机构地区：[1]贵州医科大学口腔医学院,贵州贵阳550001 [2]贵州省贵阳市口腔医院口腔颌面外科,贵州贵阳550002

出　　处：《实用临床医药杂志》2023年第14期1-8,共8页Journal of Clinical Medicine in Practice

基　　金：2022年贵州省卫生健康委科学技术基金项目(gzwkj2022-431)。

摘　　要：目的探索淫羊藿素对人舌鳞癌细胞CAL-27的增殖、凋亡、迁移和侵袭的影响及可能作用机制。方法分别采用0、10、20、40μmol/L浓度的淫羊藿素干预人舌鳞癌细胞CAL-27。采用CCK-8法及克隆形成实验检测细胞增殖;采用划痕实验检测细胞迁移;采用流式细胞术检测细胞凋亡;采用Transwell实验检测细胞迁移和侵袭。采用分子对接预测淫羊藿素与舌鳞癌目的基因结合效果,采用逆转录-实时定量聚合酶链反应(RT-qPCR)进行验证。结果淫羊藿素对CAL-27细胞作用24 h的半数抑制浓度(IC_(50))为33.37μmol/L,48 h IC_(50)为15.57μmol/L。与0μmol/L浓度淫羊藿素相比,40μmol/L浓度的CAL-27细胞克隆形成率降低,细胞凋亡率升高,差异有统计学意义(P<0.001)。淫羊藿素浓度增加时,CAL-27的迁移、侵袭数呈淫羊藿素剂量依赖性抑制;细胞内AR、ESR1、PRKACA、PTGS2的相对表达量呈淫羊藿素剂量依赖性减少。结论淫羊藿素可抑制人舌鳞癌细胞CAL-27的增殖、迁移、侵袭,促进细胞凋亡,其机制可能与淫羊藿素影响细胞CAL-27的AR、ESR1、PRKACA的表达有关。Objective To explore the effect of icaritin on proliferation,apoptosis,migration and invasion of human tongue squamous cell CAL-27 and its possible mechanism.Methods Human tongue squamous cell CAL-27 was treated with icaritin at concentrations of 0,10,20 and 40μmol/L,respectively.Cell proliferation was detected by CCK-8 assay and clonal formation assay;cell migration was detected by scratch test;the apoptosis was detected by flow cytometry;the Transwell assay was used to detect cell migration and invasion.Molecular docking was used to predict the binding effect of icaritin to the target gene of tongue squamous cell carcinoma,which was verified by quantitative reverse transcription-polymerase chain reaction(RT-qPCR).Results The median inhibitory concentration(IC_(50))of icaritin on CAL-27 cells was 33.37μmol/L for 24 h and 15.57μmol/L for 48 h.Compared with 0μmol/L concentration of icaritin,the clonal formation rate of CAL-27 cells at 40μmol/L concentration was significantly decreased,and the apoptosis rate was significantly increased(P<0.001).When icaritin concentration increased,the number of CAL-27 for migration and invasion was dose-dependently inhibited;the relative expression levels of AR,ESR1,PRKACA and PTGS2 decreased in a dose-dependent manner.Conclusion Icaritin can inhibit the proliferation,migration,invasion and apoptosis of human tongue squamous cell CAL-27,and the mechanism may be related to the effect of icaritin on the expression of AR,ESR1 and PRKACA in cell CAL-27.

关 键 词：淫羊藿素 舌鳞状细胞癌 增殖 凋亡 迁移 侵袭 机制 

分 类 号：R739.86[医药卫生—肿瘤] R446.1[医药卫生—临床医学]