多层生物介质传感器的特性研究  

Study on Characteristics of Multilayer Biosensor

作　　者：何立栋 李建平 魏碧倩 温建明[1] 刘浩 马继杰[1] 胡意立[1] 张昱[1] 万嫩 李柠 HE Li-Dong;LI Jian-Ping;WEI Bi-Qian;WEN Jian-Ming;LIU Hao;MA Ji-Jie;HU Yi-Li;ZHANG Yu;WAN Nen;LI Ning(College of Engineering,Zhejiang Normal University,Jinhua 321004,China;College of Information Science and Engineering,Jiaxing College,Jiaxing 314000,China;Hangzhou Innovation Institute,Beihang University,Hangzhou 310051,China)

机构地区：[1]浙江师范大学工学院,金华321004 [2]嘉兴学院信息科学与工程学院,嘉兴314000 [3]北京航空航天大学杭州创新研究院,杭州310051

出　　处：《生物化学与生物物理进展》2023年第6期1434-1442,共9页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(52105564);浙江省自然科学基金(LGF20E050001);浙江省重点研究开发项目(2021C01181);北航杭州创新研究院钱江实验室开放基金(2020-Y1-A-028)资助项目。

摘　　要：目的多层生物介质的生物传感器被广泛应用于各大领域,其检测特性对于传感器优劣的评估尤为重要。本文目的在于量化表征多层生物介质的电学特征。方法基于生物电阻抗谱技术来探究多层生物介质的电化学阻抗谱特性,并结合保角映射的方法来量化表征多层生物介质,阐明其对阻抗的影响规律,继而为生物传感器的研制与开发提供理论基础。有效提取各生物介质层修饰后电阻抗参数(Z^(*)),从而量化表征多层生物介质层的电阻抗谱特性。结果对多层模型进行了理论计算并构建了相关试验测试系统,研究结果表明,随着生物介质层的逐步修饰,检测区域电阻抗参数(Z^(*))在f=0.1~50 MHz下持续上升,理论计算结果趋势与试验结果趋势较好吻合,论证了此理论计算方法的正确性。结论本文证实了可根据生物电阻抗谱和保角映射方法量化表征多层生物介质的电阻抗谱特性,对生物传感器的研制与开发有一定的实用价值。Objective Biosensors with multilayer biomedia are widely applied in various fields,and quantitative characterization of biosensors is still a problem for the development of sensors.This study is to quantitatively characterize the electrical properties of multilayer biomedia.Methods Combined with conformal mapping theory,the quantitative characteristics of biosensors are explored based on electrical impedance spectroscopy for clarifying the law of influence on impedance,and this study provides a basic theory for the characterization of biosensors.The impedance(Z^(*))of each biomedia layer is extracted,and the simulation and calculation are executed to study the correctness.Results An experimental system has been established,results show that the impedance(Z^(*))of the detection area continues to rise from the frequency(f)=0.1 MHz to f=50.0 MHz in the coating process.This trend is explained that the solution in the original detection area is covered by the coating of biological medium with different dielectric properties,resulting in a decrease in the conductivity of the detection area and an increase in the impedance.Theoretical calculation results and simulation results show a great agreement with experimental results.Conclusion This study confirms that the multilayer biosensors are able to be quantitatively characterized based on electrical impedance spectroscopy and conformal mapping,which has certain practical value for the further development of biosensors.

关 键 词：生物传感器 多层生物介质 生物电阻抗谱 保角映射 阻抗 

分 类 号：R2-0[医药卫生—中医学]