装饰短蚋线粒体全基因组序列测定与分析  

Sequencing and analysis of the complete mitochondrial genome sequence of Simulium Ornatum

作　　者：孙谦 杨明 温小军 SUN Qian;YANG Ming;WEN Xiaojun(Department of Biology&Key Laboratory of Medical Entomology,Basic Medicine College,Guizhou Medical University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州医科大学基础医学院生物学系暨医学昆虫重点实验室,贵州贵阳550025

出　　处：《贵州医科大学学报》2023年第8期881-887,共7页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(31860324)。

摘　　要：目的测定和注释装饰短蚋(Simulium ornatum)线粒体全基因组序列,并在线粒体基因组水平上分析短蚋亚属与蚋亚属、纺蚋亚属之间的系统发育关系。方法采用长PCR扩增加引物步移法和总DNA测序法对装饰短蚋线粒体全基因组序列进行扩增测序,应用邻接法重建装饰短蚋与其近缘物种的系统发育关系。结果装饰短蚋线粒体基因组序列全长为15640 bp,包括13个蛋白质编码基因(PCGs)、2个rRNA基因、22个tRNA基因及1个控制区(CR),A、T、C、G碱基含量分别为38.1%、36.4%、14.7%、10.8%;基于13个PCGs序列构建的系统发育关系表示为{[(S.ornatum+S.variegatum)+S.maculatum]+S.aureohirtum}+S.quinquestriatum,基于DNA条码序列构建系统发育关系表示为{[(S.ornatum+S.variegatum)+S.quinquestriatum]+S.maculatum}+S.aureohirtum。结论短蚋亚属与蚋亚属杂色蚋组(variegatum group)的亲缘关系最近,利用线粒体全基因组进行系统发育分析DNA条码序列具有更高的分辨率。Objective To sequence and interpret the complete mitochondrial(mt)genome sequence of Simulium ornatum,and to analyze the phylogenetic relationships of subgenus Odagmia,subgenus Simulium,subgenus Byssodon,and subgenus Nevermannia on the mt genome level.Methods The mt-genome sequences of S.ornatum were amplified and sequenced by long PCR amplification plus primer walking and total DNA sequencing method,and the phylogenetic relationships between S.ornatum and its close species were reconstructed by neighbor-joining method.Results The length of the complete mt-genomes of S.ornatum was 15640 bp,including 13 protein-coding genes(PCGs),2 rRNA genes,22 tRNA genes,and 1 control region(CR).The base contents of S.ornatum were 38.1%of A,36.4%of T,14.7%of C and 10.8%of G.The phylogenetic tree based on 13 PCGs was displayed as{[(S.ornatum+S.variegatum)+S.maculatum]+S.aureohirtum}+S.quinquestriatum;based on DNA barcodes,{[(S.ornatum+S.variegatum)+S.quinquestriatum]+S.maculatum}+S.aureohirtum.Conclution The subgenus Odagmia is most closely related to the variegatum group of the subgenus Simulium;the whole mt-genomes have higher resolution than DNA barcodes for phylogenetic analysis.

关 键 词：装饰短蚋 线粒体基因组 密码子 蛋白质编码基因 系统发育关系 DNA条码 控制区 

分 类 号：R384[医药卫生—医学寄生虫学]