响应面法优化油茶叶精油提取工艺  

Optimization of Extraction Process of Essential Oil from Camellia oleifera Leaves by Response Surface Methodology

作　　者：危婷 邓爱华 王云 谢鹏 王科瑞 刘大宏 WEI Ting;DENG Aihua;WANG Yun;XIE Peng;WANG Kerui;LIU Dahong(College of Life and Environment Science,Hu'nan University of Arts and Science,Changde,Hu'nan 415000,China;Hu'nan Nitoushan Camellia Development Co.,Ltd.,Changde,Hu'nan 415700,China)

机构地区：[1]湖南文理学院生命与环境科学学院,湖南常德415000 [2]湖南泥头山油茶开发有限公司,湖南常德415700

出　　处：《农产品加工》2023年第15期29-31,41,共4页Farm Products Processing

基　　金：湖南省企业科技特派员计划项目“油茶叶功能成分提取及产品开发”(2021GK5070);湖南省自然科学基金项目“超临界流体技术制备磁响应载药微球及其生物学评价”(2022JJ50249);湖南省大学生创新创业训练计划项目“基于超临界流体技术制备富硒糖萜素工艺研究”(S202210549019);常德市科技创新发展指导性计划项目“超临界流体技术制备磁响应载药微球研究”(常科函[2021]66号);常德市企业科技特派员项目(常科函[2021]32号)。

摘　　要：研究纤维素酶辅助索氏提取油茶叶中精油的工艺条件。通过单因素试验设计,对影响精油得率的酶添加量、提取时间及料液比等因素进行探究,然后利用Design Expert 8.0软件进行Box-benhnken试验设计,酶添加量、提取时间及料液比为独立变量,精油得率为响应值进行方差分析和多项式拟合回归,建立回归数学模型。结果表明,在酶添加量2%,料液比1∶40(g∶m L)下回流提取7 h,油茶叶精油得率为(1.203±0.015)%。The technological conditions of cellulase assisted soxhlet extraction of essential oil from oil tea were studied.Through the single factor experimental design,the factors affecting the yield of essential oil,such as the amount of enzyme added,extraction time and solid-liquid ratio,were explored,and then the box benhnken experimental design was carried out by using the design expert 8.0 software.The amount of enzyme added,extraction time and solid-liquid ratio were independent variables,and the yield of essential oil was the response value for variance analysis and polynomial fitting regression,and the regression mathematical model was established.The results showed that under the condition of enzyme addition of 2%and solid-liquid ratio of 1∶40(g∶mL),the yield of essential oil of Camellia oleifera was(1.203±0.015)%.

关 键 词：油茶叶 精油 纤维素酶 索氏提取法 响应面试验 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]