出生前糖皮质激素暴露对子代大鼠情感行为障碍影响的研究  

Study on the effect of prenatal glucocorticoid exposure on affective and behavioral disorders in offspring rats

作　　者：徐海艳 张承巍[2] XU Haiyan;ZHANG Chengwei(School of Nursing of Jilin University,Changchun 130021,China;The Second Hospital of Jilin University,Changchun 130041,China)

机构地区：[1]吉林大学护理学院,长春130021 [2]吉林大学第二医院,长春130041

出　　处：《长春中医药大学学报》2023年第9期985-988,共4页Journal of Changchun University of Chinese Medicine

基　　金：吉林省教育厅科学技术研究项目(JJKH20211230KJ)。

摘　　要：目的 揭示出生前暴露于糖皮质激素对子代大鼠情感行为障碍的影响。方法 将孕鼠随机分为2组,实验组在孕15.5 d,每天给予0.15 mg·mL^(-1)地塞米松0.1 mL,对照组注射0.1 mL生理盐水直至生产。记录子代成年大鼠强迫游泳实验上窜时间及不动时间;使用微透析方法检测中缝核5-HT含量和qRT-PCR方法检测Bcl-2 mRNA与Bax mRNA表达情况。结果 与对照组相比,实验组强迫游泳实验不动时间明显延长,上窜时间明显缩短(P <0.01);中缝核内5-HT的含量明显减少(P <0.01);Bcl-2/Bax mRNA比值较低(P <0.01)。结论 出生前糖皮质激素的暴露,导致了成年后子代抑郁行为的改变,这种改变可能是通过诱发中缝核神经元细胞凋亡和神经递质5-HT含量的减少引起。Objective To reveal the effects of prenatal glucocorticoid exposure on emotional and behavioral disorders in offspring rats.Methods The pregnant rats were randomly divided into the experimental group and the control group.The experimental group was treated with 0.1 mL of 0.15 mg·mL-1 dexamethasone every day in pregnancy for 15.5 days,and the control group was injected with 0.1 mL of normal saline until production.The swimming up time and the immobility time of offspring adult rats were recorded in forced swimming experiments.Microdialysis method was used to detect the content of 5-HT in raphe nucleus and qRT PCR method was used to detect the expression of Bcl-2 mRNA and Bax mRNA.Results Compared with the control group,the immobility time of the forced swimming experiment in the experimental group was significantly prolonged,and the swimming up time was significantly shortened(P<0.01);The content of 5-HT in raphe nucleus decreased significantly(P<0.01),and the ratio of Bcl-2/Bax mRNA was lower(P<0.01).Conclusion Prenatal glucocorticoid exposure leads to the change of depressive behavior in adult offspring,which may be caused by inducing apoptosis of raphe nucleus neurons and reducing the content of neurotransmitter 5-HT.

关 键 词：糖皮质激素 强迫游泳实验 B淋巴细胞瘤-2基因 

分 类 号：R741[医药卫生—神经病学与精神病学]