聚乙二醇化聚癸二酸甘油酯/β-磷酸三钙涂层改性镁合金膜的体外生物学评价  

In vitro biological evaluation of PEGylated poly(glycerol sebacate)/β-TCP-coating modified magnesium alloy

作　　者：张成龙 张昌入 司家文[1] 袁媛[2] 于洪波[1] 沈洪洲 沈国芳[1,4] ZHANG Cheng-long;ZHANG Chang-ru;SI Jia-wen;YUAN Yuan;YU Hong-bo;SHEN Hong-zhou;SHEN Guo-fang(Department of Oral and Craniomaxillofacial Surgery,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai 200011;Engineering Research Center for Biomaterials of Ministry of Education,East China University of Science and Technology.Shanghai 200237;Institute of Translational Medicine,Shanghai Jiao Tong University.Shanghai 200240;Shanghai University of Medicine&Health Sciences.Shanghai 201318,China)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔颅颌面科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011 [2]华东理工大学医用生物材料教育部工程研究中心,上海200237 [3]上海交通大学转化医学研究院,上海200240 [4]上海健康医学院,上海201318

出　　处：《上海口腔医学》2023年第4期342-350,共9页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81970973);上海交通大学医学院多中心临床研究(DLY201808)。

摘　　要：目的:通过在镁锌钆合金(MZG)膜表面形成乙二醇化聚癸二酸甘油酯/β-磷酸三钙(PEGS/β-TCP)涂层,制备PEGS/β-TCP改性镁合金(PEGS/β-TCP/MZG)膜,使用材料浸提液对PEGS/β-TCP/MZG复合膜的成骨诱导活性和免疫调节性能进行评价。方法:采用溶胶凝胶法在MZG膜表面制备PEGS/β-TCP涂层,制备PEGS/β-TCP/MZG复合膜,并与PEGS/β-TCP和MZG膜进行对比,检测其形貌、成分和亲水性。检测材料浸提3天后的镁离子释放量和pH值。体外细胞实验观察成骨细胞MC3T3-E1细胞在浸提液中的增殖活性、碱性磷酸酶(alkaline phosphatase,ALP)和矿化结节形成情况,以及RAW264.7巨噬细胞在浸提液中的增殖活性、极化和细胞内相关因子的表达。采用GraphPad Prism 9.0软件包对数据进行统计学分析。结果:制备出PEGS/β-TCP涂层与MZG膜紧密嵌合的PEGS/β-TCP/MZG复合膜,具有良好亲水性,可缓慢降解。细胞实验结果显示,PEGS/β-TCP/MZG复合膜浸提液对MC3T3-E1和巨噬细胞的增殖活性无明显影响。PEGS/β-TCP/MZG组显著增强MC3T3-E1的ALP表达和矿化结节形成,PEGS/β-TCP组和PEGS/β-TCP/MZG组在巨噬细胞极化模式上虽无明显差异,但PEGS/β-TCP/MZG膜在PEGS/β-TCP涂层的免疫调节基础上,可进一步降低炎症相关肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)表达,提高成骨相关转化生长因子β(transforming growth factor-β,TGF-β)表达。结论:PEGS/β-TCP复合膜改性的MZG可为骨组织工程的发展提供新的材料选择。PURPOSE: To prepare PEGS/β-TCP modified magnesium alloy (PEGS/β-TCP/MZG) membranes by forming a glycolated poly(sebacate)/β-tricalcium phosphate (PEGS/β-TCP) coating on the surface of magnesium-zinc-gadolinium alloy (MZG) membranes, and to evaluate the osteogenic induction activity and immunomodulatory properties of PEGS/β-TCP/MZG using the material extract medium. METHODS: PEGS/β-TCP coating was prepared on the surface of MZG by solvent method, and the PEGS/β-TCP/MZG membrane was fabricated and compared with PEGS/β-TCP and MZG to examine the morphology, composition, and hydrophilicity. The amount of magnesium ions released and the pH value of the materials were tested after 3 days of immersion. The cell viability and osteogenic differentiation of MC3T3 cells induced by extract medium were investigated by CCK-8 assay, ALP and mineralized nodule staining. The cell viability and polarization of RAW cells induced by extract medium were then investigated. The expression of macrophage-secreted cytokines was examined by PCR analysis. GraphPad Prism 9.0 software package was used for statistical analysis. RESULTS: PEGS/β-TCP/MZG membranes with PEGS/β-TCP coating tightly embedded with MZG were successfully fabricated, and the material had good hydrophilicity. The results of degradation experiments indicated that the PEGS/β-TCP coating effectively slowed down the degradation rate of MZG, leading to a lower pH value and concentration of Mg2+ ion in the extract medium of PEGS/β-TCP/MZG group. The results of in vitro cell experiments showed that PEGS/β-TCP/MZG had no significant effect on the proliferation activity of both MC3T3-E1 and macrophages. PEGS/β-TCP/MZG significantly enhanced the expression of ALP and mineralized nodule staining in MC3T3-E1. Although there was no significant difference in macrophage polarization pattern between PEGS/β-TCP and PEGS/β-TCP/MZG groups, PEGS/β-TCP/MZG further reduced inflammation based on the immunomodulation of PEGS/β-TCP coating related TNF-α expression and increased

关 键 词：镁合金 PEGS/β-TCP 骨再生 免疫调节 

分 类 号：R783.1[医药卫生—口腔医学]