circRNA-ZNF532、circRNA-HIPK3与糖尿病视网膜病变的相关性研究  

Correlation analysis of circRNA-ZNF532 and circRNA-HIPK3 with diabetic retinopathy

作　　者：隋源 张成森 王爽 李雪丽 勾晓梅 SUI Yuan;ZHANG Chengsen;WANG Shuang;LI Xueli;GOU Xiaomei(Department of Ophthalmology,Harbin 242 Hospital,Harbin 150000,China)

机构地区：[1]哈尔滨二四二医院眼科,150000

出　　处：《天津医药》2023年第9期993-997,共5页Tianjin Medical Journal

摘　　要：目的探讨环状RNA(circRNA)-锌指蛋白532(ZNF532)、circRNA-同源域相互作用蛋白激酶3(HIPK3)与糖尿病视网膜病变(DR)的相关性。方法纳入109例DR患者(DR组)、110例单纯糖尿病患者(DM组)和65例健康体检志愿者(对照组)。实时荧光定量聚合酶链反应(qPCR)检测血清circRNA-ZNF532、circRNA-HIPK3表达,酶联免疫吸附试验检测血清血管内皮生长因子(VEGF)、白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α、IL-6水平。Pearson分析DR患者血清circRNA-ZNF532、circRNA-HIPK3表达与血清VEGF、IL-1β、TNF-α、IL-6水平相关性。单因素和多因素Logistic回归分析DR危险因素,受试者工作特征(ROC)曲线分析circRNA-ZNF532、circRNA-HIPK3诊断DR的价值。结果DR组血清circRNA-ZNF532、circRNA-HIPK3表达以及VEGF、IL-1β、TNF-α、IL-6水平高于DM组和对照组(P<0.05)。DR组血清circRNA-ZNF532、circRNA-HIPK3表达与VEGF、IL-1β、TNF-α、IL-6水平均呈正相关(P<0.05)。多因素Logistic回归分析结果显示高水平circRNA-ZNF532、circRNA-HIPK3及VEGF是糖尿病患者发生DR的独立危险因素(P<0.05)。三者诊断糖尿病患者发生DR的曲线下面积分别为0.736、0.740和0.864,联合诊断高于单独诊断(P<0.05)。结论糖尿病患者血清中circRNA-ZNF532、circRNA-HIPK3表达上调与DR发生有关,两者可以作为DR辅助诊断的潜在指标。Objective To investigate the correlation between circrNa-Zinc finger protein 532(ZNF532),circrNahomologous domain interaction protein kinase-3(HIPK3)and diabetic retinopathy(DR).Methods A total of 109 patients with DR(DR group),110 patients with simple diabetes(DM group)and 65 healthy volunteers(control group)were selected from our hospital from October 2020 to October 2022.Serum circRNA-ZNF532 and circRNA-HIPK3 expression levels were detected by real-time quantitative polymerase chain reaction(RT-PCR),and serum vascular endothelial growth factor(VEGF),interleukin(IL)-1β,tumor necrosis factor(TNF)-αand IL-6 levels were detected by enzyme-linked immunosoradsorption assay.The correlation between serum circRNA-ZNF532 and circRNA-HIPK3 expression and serum levels of VEGF,IL-1β,TNF-αand IL-6 were analyzed by Pearson method.Univariate Logistic regression analysis and multivariate Logistic regression analysis were used for DR risk factors,and receiver operating characteristic curve(ROC)was used for analysis of circRNA-ZNF532 and circRNA-HIPK3 diagnostic value of DR.Results Serum expressions of circRNA-ZNF532 and circRNA-HIPK3 and levels of VEGF,IL-1β,TNF-αand IL-6 were significantly higher in the DR group than those in the DM group and the control group(P<0.05).Serum circRNA-ZNF532 and circRNA-HIPK3 expression were positively correlated with levels of VEGF,IL-1β,TNF-αand IL-6 in the DR group(P<0.05).Multivariate Logistic regression analysis showed that high levels of VEGF,circRNA-ZNF532 and circRNA-HIPK3 were risk factors for developing DR in diabetic patients(P<0.05).The areas under the curve of circRNA-ZNF532,circRNA-HIPK3 and VEGF in the diagnosis of DR in diabetic patients were 0.736,0.740 and 0.864.Combined diagnosis was higher than that of circRNA-ZNF532,circRNA-HIPK3 and VEGF alone(P<0.05).Conclusion The up-regulated expression levels of circRNA-ZNF532 and circRNA-HIPK3 in serum of diabetic patients are related to the occurrence of DR,and both circRNA-ZNF532 and circRNA-HIPK3 can be used as potential indi

关 键 词：糖尿病视网膜病变 RNA 环状 转录激活因子类 tristetraprolin蛋白 同源盒结构域蛋白质类 炎症 血管内皮生长因子类 同源域相互作用蛋白激酶3 

分 类 号：R774.13[医药卫生—眼科]