甜叶菊药材品质与质量提升研究  

Research on improvement of the qualities of Stevia rebaudiana(bertoni)hemsl.

作　　者：赵向阳 余秀萍 吴玮 崔灿 孙婷婷 郭明飞 ZHAO Xiangyang;YU Xiuping;WU Wei;CUI Can;SUN Tingting;GUO Mingfei(Anhui Suzhou Food and Drug Inspection and Testing Center,Suzhou,Anhui 234000,China;Suzhou Administration for Market Regulation,Suzhou,Anhui 234000,China)

机构地区：[1]安徽省宿州市食品药品检验检测中心,安徽宿州234000 [2]宿州市市场监督管理局,安徽宿州234000

出　　处：《安徽医药》2023年第10期1937-1940,I0002,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立甜叶菊的质量标准研究方法。方法在2020年10月至2021年12月,参照2020年版《中国药典(四部)》,对药材样品的显微特征、水分、总灰分、酸不溶性灰分、醇溶性浸出物进行检测;采用薄层色谱(TLC)法对药材样品进行定性鉴别;采用高效液相色谱(HPLC)法测定药材样品中甜菊苷和莱苞迪苷A的含量,色谱柱为Shim-pack GIST(4.6 mm×250 mm,5μm),流动相为乙腈-水(35∶65),流速为0.5 mL/min,检测波长为210 nm,柱温为40℃,进样量为10μL。结果药材样品粉末呈暗绿色或黄绿色,显微特征清晰。TLC图斑点清晰,且分离度好。甜菊苷和莱苞迪苷A分别在0.10~2.01 g/L(r=0.9997)和0.10~1.17 g/L(r=0.9999)范围内与峰面积积分值线性关系良好;精密度、稳定性、重复性试验结果的RSD均小于2.00%;平均加样回收率分别为98.5%和98.4%,RSD分别为0.30%和0.45%(n=6)。10批药材样品的平均水分、总灰分、酸不溶性灰分含量分别为4.0%、7.2%和0.9%,醇溶性浸出物含量均大于50%;甜菊苷和莱苞迪苷A平均含量分别为11.4%和4.1%。结论所建标准可用于甜叶菊的质量控制。Objective To establish a method for the study of quality standards of Stevia rebaudiana(bertoni)hemsl.Methods Between October 2020 and 2021 December,the microscopic characteristics,moisture content,total ash,acid-insoluble ash,and alcoholsoluble extracts of the Stevia rebaudiana(Bertoni)Hemsl.were determined according to the Chinese Pharmacopoeia(2020 Edition,VolumeⅣ).Thin-layer chromatography(TLC)was used to qualitatively identify Stevia rebaudiana(bertoni)hemsl.High-performance liquid chromatography(HPLC)method was used to determine the content of stevioside and rebaudioside A in Stevia rebaudiana(bertoni)hemsl.The chromatographic column was Shim-pack GIST column(4.6 mm×250 mm,5μm),the mobile phase was acetonitrile-water(35∶65,V/V),the flow rate was 0.5 mL/min,the detection wavelength was 210 nm,the column temperature was 40℃,and the injection volume was 10μL.Results The sample powder was dark green or chartreuse,with clear microscopic characteristics.The TLC spots were clear with good separation.The linear ranges of stevioside and rebaudioside A were 0.10-2.01 g/L(r=0.9997)and 0.10-1.17 g/L(r=0.9999),respectively.The RSDs of precision,stability and repeatability tests were less than 2.00%.The average recoveries of stevioside and rebaudioside A were 98.5%and 98.4%,with RSDs of 0.30%and 0.45%(n=6),respectively.The average content of moisture,total ash and acid-insoluble ash of the ten batches of samples was 4.0%,7.2%and 0.9%,respectively,and their alcohol-soluble extract contents were more than 50%.The average content of stevioside and rebaudioside A was 11.4%and 4.1%,respectively.Conclusion The established standard can be used for the quality control of decoction slices of Stevia rebaudiana(bertoni)hemsl.

关 键 词：甜菊属 主成分分析 薄层色谱 高效液相色谱(HPLC) 甜菊苷 莱苞迪苷A 

分 类 号：R284[医药卫生—中药学]