新疆伽师县甜瓜病毒病种类鉴定  

Identification of Virus Disease Types of Melon Planting in Jiashi County of Xinjiang

作　　者：王东 麦麦提艾则孜·穆合塔尔 刘艳全 迪力达尔克孜·吾拉木 WANG Dong;MAIMAITIAIZEZI·Muhetaer;LIU Yanquan;and DILIDAERKEZI·Wulamu(Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region,College of Life and Geographic Sciences,Kashi University,Kashi,Xinjiang 844000,China)

机构地区：[1]喀什大学生命与地理科学学院,新疆帕米尔高原生物资源与生态重点实验室,新疆喀什844000

出　　处：《园艺学报》2023年第8期1793-1802,共10页Acta Horticulturae Sinica

基　　金：新疆自然科学基金项目(2019D01B02)。

摘　　要：于2019和2021年对新疆伽师县12个乡镇进行厚皮甜瓜(通常称伽师瓜)病毒病进行调研采样,采用两步法RT-PCR鉴定西瓜花叶病毒(watermelon mosaic virus,WMV)、黄瓜花叶病毒(cucumber mosaicvirus,CMV)、小西葫芦黄化花叶病毒(zucchiniyellowmosaicvirus,ZYMV)、黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)、南瓜蚜传黄化病毒(cucurbit aphid-borne yellows virus,CABYV)和甜瓜蚜传黄化病毒(melon aphid-borne yellows virus,MABYV),并对WMV基因组进行分析。结果表明,伽师瓜病毒病发生较普遍,症状包括花叶、畸形、坏死和褪绿黄化等。采集的37份样品中,病毒的检出率为21.6%(8/37),其中WMV的检出率最高,为18.9%(7/37),CMV和ZYMV次之,没有鉴定到CGMMV、CABYV和MABYV,复合侵染更普遍。不同WMV分离物的F2/R2(P1+HC-Pro)、F4/R4(P3+6K1+CI)、F5/R5(CI+6K2+NIa-VPg)、F6/R6(6K2+NIa-VPg+NIa-Pro+NIb)和F8/R8(CP+3′-UTR)等基因组片段在进化树上的分支具有一定的差异,表明WMV基因组可能存在重组现象。In 2019 and 2021,12 towns in Jiashi county of Xinjiang were investigated and sampled for muskmelon(Jiashi-melon)virus disease.Watermelon mosaic virus(WMV),cucumber mosaic virus(CMV),zucchini yellow mosaic virus(ZYMV),cucumber green mottle mosaic virus(CGMMV),cucurbit aphid-borne yellows virus(CABYV),and melon aphid-borne yellows virus(MABYV)were identified by two-step RT-PCR protocol.In addition,the genome of WMV isolates was analyzed.The results showed that the virus disease is more common in the Jiashi-melon planting towns in Jiashi County.Symptoms include mosaic,blister mosaic,blister mosaic and malformation,leaf necrosis,leaf chlorosis,and fruit malformation.A total of 37 samples were collected,and the detection of WMV,CMV,and ZYMV was positive,but CGMMV,CABYV,and MABYV were not detected.21.6%(8/37)of the samples were positive.WMV was the most prevalent virus detected in 18.9%(7/37)of the 37 samples collected,followed by CMV and ZYMV.Multiple infections were more frequent than single infections.F2/R2(P1+HC-Pro),F4/R4(P3+6K1+CI),F5/R5(CI+6K2+NIa-VPg),F6/R6(6K2+NIa-VPg+NIa-Pro+NIb),and F8/R8(CP+3′-UTR)fragments of different WMV isolates infecting Jiashi-melon have certain differences in the branches of the evolutionary tree,indicating that there may be recombination in the WMV genome.

关 键 词：甜瓜 病毒鉴定 基因组分析 西瓜花叶病毒 

分 类 号：S652.1[农业科学—果树学]