Comparative transcriptome and lipidome reveal that a low K^(+) signal effectively alleviates the effect induced by Ca^(2+) deficiency in cotton fibers  

作　　者：GUO Kai GAO Wei ZHANG Tao-rui WANG Zu-ying SUN Xiao-ting YANG Peng LONG Lu LIU Xue-ying WANG Wen-wen TENG Zhong-hua LIU Da-jun LIU De-xin TU Li-li ZHANG Zheng-sheng 

机构地区：[1]College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,P.R.China [2]State Key Laboratory of Cotton Biology/Henan Key Laboratory of Plant Stress Biology/School of Life Sciences,Henan University,Kaifeng475001,P.R.China [3]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第8期2306-2322,共17页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31901577);the Fundamental Research Funds for the Central Universities,China(SWU-KT22035);the State Key Laboratory of Cotton Biology Open Fund,China(CB2021A32)。

摘　　要：Calcium(Ca^(2+))plays an important role in determining plant growth and development because it maintains cell wall and membrane integrity.Therefore,understanding the role of Ca^(2+)in carbon and lipid metabolism could provide insights into the dynamic changes in cell membranes and cell walls during the rapid elongation of cotton fibers.In the present study,we found that the lack of Ca^(2+)promoted fiber elongation and rapid ovule expansion,but it also caused tissue browning in the ovule culture system.RNA-sequencing revealed that Ca^(2+)deficiency induced cells to be highly oxidized,and the expression of genes related to carbon metabolism and lipid metabolism was activated significantly.All gene members of nine key enzymes involved in glycolysis were up-regulated,and glucose was significantly reduced in Ca^(2+)deficiency-treated tissues.Ca^(2+)deficiency adjusted the flowing of glycolysis metabolic.However,low K^(+)recovered the expression levels of glycolysis genes and glucose content caused by Ca^(2+)deficiency.Electrospray ionizationtandem mass spectrometry technology was applied to uncover the dynamic profile of lipidome under Ca^(2+)and K^(+)interacted conditions.Ca^(2+)deficiency led to the decrease of fatty acid(FA),diacylglycerol(DAG),glycolipid and the significant increase of triacylglycerol(TAG),phospholipid phosphatidylethanolamine(PE),phosphatidylglycerol(PG),and PC(phosphatidylcholine).Low K^(+)restored the contents of FA,phospholipids,and glycolipids,effectively relieved the symptoms caused by Ca^(2+)deficiency,and recovered the development of fiber cells.This study revealed dynamic changes in transcript and metabolic levels and uncovered the signaling interaction of Ca^(2+)deficiency and low K^(+)in glycolysis and lipid metabolism during fiber development.

关 键 词：cotton fiber GLYCOLYSIS LIPIDOME calcium potassium 

分 类 号：S562[农业科学—作物学]